Quantitative technologies establish a novel microRNA profile of chronic lymphocytic leukemia Journal Article
| Authors: | Fulci, V.; Chiaretti, S.; Goldoni, M.; Azzalin, G.; Carucci, N.; Tavolaro, S.; Castellano, L.; Magrelli, A.; Citarella, F.; Messina, M.; Maggio, R.; Peragine, N.; Santangelo, S.; Mauro, F. R.; Landgraf, P.; Tuschl, T.; Weir, D. B.; Chien, M.; Russo, J. J.; Ju, J.; Sheridan, R.; Sander, C.; Zavolan, M.; Guarini, A.; Foà, R.; Macino, G. |
| Article Title: | Quantitative technologies establish a novel microRNA profile of chronic lymphocytic leukemia |
| Abstract: | MicroRNAs (miRNAs) are a novel class of small noncoding RNAs that modulate the expression of genes at the posttranscriptional level. These small molecules have been shown to be involved in cancer, apoptosis, and cell metabolism. In the present study we provide an informative profile of the expression of miRNAs in primary chronic lymphocytic leukemia (CLL) cells using 2 independent and quantitative methods: miRNA cloning and quantitative real-time-polymerase chain reaction (qRT-PCR) of mature miRNAs. Both approaches show that miR-21 and miR-155 are dramatically overexpressed in patients with CLL, although the corresponding genomic loci are not amplified. miR-150 and miR-92 are also significantly deregulated in patients with CLL. In addition, we detected a marked miR-15a and miR-16 decrease in about 11% of cases. Finally, we identified a set of miRNAs whose expression correlates with biologic parameters of prognostic relevance, particularly with the mutational status of the IgV H genes. In summary, the results of this study offer for the first time a comprehensive and quantitative profile of miRNA expression in CLL and their healthy counterpart, suggesting that miRNAs could play a primary role in the disease itself. © 2007 by The American Society of Hematology. |
| Keywords: | controlled study; human tissue; human cell; major clinical study; in situ hybridization, fluorescence; gene overexpression; apoptosis; microrna; gene expression; gene expression profiling; molecular dynamics; genetic transcription; molecular cloning; cloning, molecular; reverse transcriptase polymerase chain reaction; oligonucleotide array sequence analysis; quantitative analysis; gene identification; genomics; genome; lymphatic leukemia; dna mutational analysis; micrornas; genetic techniques; cell metabolism; zap-70 protein-tyrosine kinase; rna processing, post-transcriptional; immunoglobulins; leukemia, lymphocytic, chronic |
| Journal Title: | Blood |
| Volume: | 109 |
| Issue: | 11 |
| ISSN: | 0006-4971 |
| Publisher: | American Society of Hematology |
| Date Published: | 2007-06-01 |
| Start Page: | 4944 |
| End Page: | 4951 |
| Language: | English |
| DOI: | 10.1182/blood-2006-12-062398 |
| PUBMED: | 17327404 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 169" - "Export Date: 17 November 2011" - "CODEN: BLOOA" - "Source: Scopus" |
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