miRNome profiling reveals shared features in breast cancer subtypes and highlights miRNAs that potentially regulate MYB and EZH2 expression Journal Article

   

Authors:	Corrêa, S.; Lopes, F. P.; Panis, C.; Basili, T.; Binato, R.; Abdelhay, E.
Article Title:	miRNome profiling reveals shared features in breast cancer subtypes and highlights miRNAs that potentially regulate MYB and EZH2 expression
Abstract:	Breast cancer (BC) has been extensively studied, as it is one of the more commonly diagnosed cancer types worldwide. The study of miRNAs has increased what is known about the complexity of pathways and signaling and has identified potential biomarkers and therapeutic targets. Thus, miRNome profiling could provide important information regarding the molecular mechanisms involved in BC. On average, more than 430 miRNAs were identified as differentially expressed between BC cell lines and normal breast HMEC cells. From these, 110 miRNAs were common to BC subtypes. The miRNome enrichment analysis and interaction maps highlighted epigenetic-related pathways shared by all BC cell lines and revealed potential miRNA targets. Quantitative evaluation of BC patient samples and GETx/TCGA-BRCA datasets confirmed MYB and EZH2 as potential targets from BC miRNome. Moreover, overall survival was impacted by EZH2 expression. The expression of 15 miRNAs, selected according to aggressiveness of BC subtypes, was confirmed in TCGA-BRCA dataset. Of these miRNAs, miRNA-mRNA interaction prediction revealed 7 novel or underexplored miRNAs in BC: miR-1271-5p, miR-130a-5p, and miR-134 as MYB regulators and miR-138-5p, miR-455-3p, miR-487a, and miR-487b as EZH2 regulators. Herein, we report a novel molecular miRNA signature for BC and identify potential miRNA/mRNAs involved in disease subtypes. © Copyright © 2021 Corrêa, Lopes, Panis, Basili, Binato and Abdelhay.
Keywords:	signal transduction; cancer survival; controlled study; human tissue; protein expression; cell proliferation; cell survival; breast cancer; microrna; gene expression; cohort analysis; angiogenesis; carcinogenesis; epigenetics; quantitative analysis; clinical evaluation; cell migration; down regulation; real time polymerase chain reaction; upregulation; penicillin derivative; dna extraction; protein c myb; glutamine; transcription factor ezh2; streptomycin; ezh2; human; female; article; breast cancer cell line; myb; protein fingerprinting; mirnome; novel mirnas; hmec cell line (microvascular endothelium)
Journal Title:	Frontiers in Oncology
Volume:	11
ISSN:	2234-943X
Publisher:	Frontiers Media S.A.
Date Published:	2021-09-27
Start Page:	710919
Language:	English
DOI:	10.3389/fonc.2021.710919
PROVIDER:	scopus
PMCID:	PMC8502886
PUBMED:	34646766
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-85116917259&doi=10.3389%2ffonc.2021.710919&partnerID=40&md5=74f34817d0790387a0df9eb8dda07129
Notes:	Article -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/188768