A single miRNA-mRNA interaction affects the immune response in a context-and cell-type-specific manner Journal Article
| Authors: | Lu, L. F. ; Gasteiger, G.; Yu, I. S.; Chaudhry, A.; Hsin, J.; Lu, Y.; Bos, P. D.; Lin, L. L.; Zawislak, C. L.; Cho, S.; Sun, J. C.; Leslie, C. S.; Lin, S. W.; Rudensky, A. Y. |
| Article Title: | A single miRNA-mRNA interaction affects the immune response in a context-and cell-type-specific manner |
| Abstract: | MicroRNA (miRNA)-dependent regulation of gene expression confers robustness to cellular phenotypes and controls responses to extracellular stimuli. Although a single miRNA can regulate expression of hundreds of target genes, it is unclear whether any of its distinct biological functions can be due to the regulation of a single target. To explore invivo the function of a single miRNA-mRNA interaction, we mutated the 3' UTR of a major miR-155 target (SOCS1) to specifically disrupt its regulation by miR-155. We found that under physiologic conditions and during autoimmune inflammation or viral infection, some immunological functions of miR-155 were fully or largely attributable to the regulation of SOCS1, whereas others could be accounted only partially or not at all by this interaction. Our data suggest that the role of a single miRNA-mRNA interaction is dependent on cell type and biological context. © 2015 Elsevier Inc. |
| Keywords: | controlled study; nonhuman; comparative study; animal cell; mouse; interleukin 2; gene expression; gene expression profiling; gene function; in vivo study; cell differentiation; gene expression regulation; regulatory t lymphocyte; cellular immunity; gamma interferon; antigen specificity; messenger rna; gene disruption; gene repression; autoimmunity; virus infection; 3' untranslated region; molecular interaction; interleukin 17; th17 cell; cd4 antigen; autoimmune disease; stat5 protein; immunocompetent cell; microrna 155; suppressor of cytokine signaling 1; interactions with rna; priority journal; article; cells by body anatomy |
| Journal Title: | Immunity |
| Volume: | 43 |
| Issue: | 1 |
| ISSN: | 1074-7613 |
| Publisher: | Cell Press |
| Date Published: | 2015-07-21 |
| Start Page: | 52 |
| End Page: | 64 |
| Language: | English |
| DOI: | 10.1016/j.immuni.2015.04.022 |
| PROVIDER: | scopus |
| PMCID: | PMC4529747 |
| PUBMED: | 26163372 |
| DOI/URL: | |
| Notes: | Export Date: 2 September 2015 -- Source: Scopus |
