The effect of cellular context on miR-155-mediated gene regulation in four major immune cell types Journal Article
| Authors: | Hsin, J. P.; Lu, Y.; Loeb, G. B.; Leslie, C. S.; Rudensky, A. Y. |
| Article Title: | The effect of cellular context on miR-155-mediated gene regulation in four major immune cell types |
| Abstract: | Numerous microRNAs and their target mRNAs are coexpressed across diverse cell types. However, it is unknown whether they are regulated in a manner independent of or dependent on cellular context. Here, we explored transcriptome-wide targeting and gene regulation by miR-155, whose activation-induced expression plays important roles in innate and adaptive immunity. Through mapping of miR-155 targets through differential iCLIP, mRNA quantification with RNA-seq, and 3′ untranslated region (UTR)-usage analysis with poly(A)-seq in macrophages, dendritic cells, and T and B lymphocytes either sufficient or deficient in activated miR-155, we identified numerous targets differentially bound by miR-155. Whereas alternative cleavage and polyadenylation (ApA) contributed to differential miR-155 binding to some transcripts, in most cases, identical 3′-UTR isoforms were differentially regulated across cell types, thus suggesting ApA-independent and cellular-context-dependent miR-155-mediated gene regulation. Our study provides comprehensive maps of miR-155 regulatory networks and offers a valuable resource for dissecting context-dependent and context-independent miRNA-mediated gene regulation in key immune cell types. © 2018, The Author(s), under exclusive licence to Springer Nature America, Inc. |
| Journal Title: | Nature Immunology |
| Volume: | 19 |
| Issue: | 10 |
| ISSN: | 1529-2908 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2018-10-01 |
| Start Page: | 1137 |
| End Page: | 1145 |
| Language: | English |
| DOI: | 10.1038/s41590-018-0208-x |
| PUBMED: | 30224821 |
| PROVIDER: | scopus |
| PMCID: | PMC6158091 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 November 2018 -- Source: Scopus |
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