Selective lysosome membrane turnover is induced by nutrient starvation Journal Article

   


Authors: Lee, C.; Lamech, L.; Johns, E.; Overholtzer, M.
Article Title: Selective lysosome membrane turnover is induced by nutrient starvation
Abstract: Lee et al. show that autophagy proteins regulate lysosome size and function in response to metabolic stress by controlling selective turnover of the lysosomal membrane. Selective turnover occurs through microautophagy activity that is regulated by core autophagy proteins and is associated with direct lipidation of LC3 onto the lysosomal membrane. © 2020 Elsevier Inc. Lysosome function is essential for cellular homeostasis, but quality-control mechanisms that maintain healthy lysosomes remain poorly characterized. Here, we developed a method to measure lysosome turnover and use this to identify a selective mechanism of membrane degradation that involves lipidation of the autophagy protein LC3 onto lysosomal membranes and the formation of intraluminal vesicles through microautophagy. This mechanism is induced in response to metabolic stress resulting from glucose starvation or by treatment with pharmacological agents that induce osmotic stress on lysosomes. Cells lacking ATG5, an essential component of the LC3 lipidation machinery, show reduced ability to regulate lysosome size and degradative capacity in response to activation of this mechanism. These findings identify a selective mechanism of lysosome membrane turnover that is induced by stress and uncover a function for LC3 lipidation in regulating lysosome size and activity through microautophagy. © 2020 Elsevier Inc.
Keywords: metabolism; glucose; autophagy; glutamine; lysosome; lc3; atg5; ammonium; lap; microautophagy
Journal Title: Developmental Cell
Volume: 55
Issue: 3
ISSN: 1534-5807
Publisher: Cell Press  
Date Published: 2020-11-09
Start Page: 289
End Page: 297.e4
Language: English
DOI: 10.1016/j.devcel.2020.08.008
PUBMED: 32916093
PROVIDER: scopus
PMCID: PMC8337093
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85094964947&doi=10.1016%2fj.devcel.2020.08.008&partnerID=40&md5=86fa0201ae3c11e1a6d08e1a47ab3263
Notes: Article -- Export Date: 1 December 2020 -- Source: Scopus
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MSK Authors
  1. Michael H Overholtzer
    81 Overholtzer
  2. Lilian Tawsein Malvika Lamech
    5 Lamech
  3. Chan Lee
    4 Lee
  4. Eleanor May Johns
    6 Johns
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