Getting picky with the lysosome membrane Journal Article


Authors: Lee, C.; Overholtzer, M.
Article Title: Getting picky with the lysosome membrane
Abstract: Lysosomes play an essential role in quality control mechanisms by functioning as the primary digestive system in mammalian cells. However, the quality control mechanisms governing healthy lysosomes are not fully understood. Using a method to study lysosome membrane turnover, we discovered that LC3-lipidation on the lysosome limiting membrane is involved in invagination and formation of intralumenal vesicles, an activity known as microautophagy. This activity occurs in response to metabolic stress, in the form of glucose starvation, or osmotic stress induced by treatment with lysosomotropic compounds. Cells rendered deficient in the ability to lipidate LC3 through knockout of ATG5 show reduced ability to regulate lysosome size and degradative function in response to stress. These findings demonstrate that cells can adapt to changing metabolic conditions by turning over selective portions of the lysosomal membrane, using a mechanism that involves lysosome-targeted LC3 lipidation and the induction of selective microautophagy. © 2021 Informa UK Limited, trading as Taylor & Francis Group.
Keywords: autophagy; lc3; atg5; microautophagy; lysophagy
Journal Title: Autophagy
Volume: 17
Issue: 4
ISSN: 1554-8627
Publisher: Taylor & Francis Group  
Date Published: 2021-04-01
Start Page: 1034
End Page: 1036
Language: English
DOI: 10.1080/15548627.2021.1877935
PUBMED: 33499722
PROVIDER: scopus
PMCID: PMC8078747
DOI/URL:
Notes: Article -- Export Date: 1 June 2021 -- Source: Scopus
Altmetric
Citation Impact
BMJ Impact Analytics
MSK Authors
  1. Chan Lee
    4 Lee