Single-cell transcriptomics reveals early emergence of liver parenchymal and non-parenchymal cell lineages Journal Article

   

Authors:	Lotto, J.; Drissler, S.; Cullum, R.; Wei, W.; Setty, M.; Bell, E. M.; Boutet, S. C.; Nowotschin, S.; Kuo, Y. Y.; Garg, V.; Pe'er, D.; Church, D. M.; Hadjantonakis, A. K.; Hoodless, P. A.
Article Title:	Single-cell transcriptomics reveals early emergence of liver parenchymal and non-parenchymal cell lineages
Abstract:	The cellular complexity and scale of the early liver have constrained analyses examining its emergence during organogenesis. To circumvent these issues, we analyzed 45,334 single-cell transcriptomes from embryonic day (E)7.5, when endoderm progenitors are specified, to E10.5 liver, when liver parenchymal and non-parenchymal cell lineages emerge. Our data detail divergence of vascular and sinusoidal endothelia, including a distinct transcriptional profile for sinusoidal endothelial specification by E8.75. We characterize two distinct mesothelial cell types as well as early hepatic stellate cells and reveal distinct spatiotemporal distributions for these populations. We capture transcriptional profiles for hepatoblast specification and migration, including the emergence of a hepatomesenchymal cell type and evidence for hepatoblast collective cell migration. Further, we identify cell-cell interactions during the organization of the primitive sinusoid. This study provides a comprehensive atlas of liver lineage establishment from the endoderm and mesoderm through to the organization of the primitive sinusoid at single-cell resolution. © 2020 Elsevier Inc. A comprehensive atlas of mouse liver emergence is described at single-cell resolution starting at endoderm progenitor specification, including data detailing divergence of vascular and sinusoidal endothelia, hepatoblast specification, and the emergence of a distinct, migratory hepatomesenchymal cell type. © 2020 Elsevier Inc.
Keywords:	controlled study; nonhuman; animal cell; mouse; mesenchyme cell; embryo; cell population; transcriptomics; cell lineage; liver; vascular endothelium; stellate cell; cell migration; liver parenchyma; cell interaction; mesoderm; endoderm; organogenesis; intracellular signaling; stellate cells; single cell analysis; mesothelium; single-cell; liver development; priority journal; article; sinusoidal endothelium; hepatoblasts; scrna-seq
Journal Title:	Cell
Volume:	183
Issue:	3
ISSN:	0092-8674
Publisher:	Cell Press
Date Published:	2020-10-29
Start Page:	702
End Page:	716.e14
Language:	English
DOI:	10.1016/j.cell.2020.09.012
PUBMED:	33125890
PROVIDER:	scopus
PMCID:	PMC7643810
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-85094212696&doi=10.1016%2fj.cell.2020.09.012&partnerID=40&md5=7228416dc8190a93996713ef42221f50
Notes:	Article -- Export Date: 1 December 2020 -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/173133