Asynchronous fate decisions by single cells collectively ensure consistent lineage composition in the mouse blastocyst Journal Article

   

Authors:	Saiz, N.; Williams, K. M.; Seshan, V. E.; Hadjantonakis, A. K.
Article Title:	Asynchronous fate decisions by single cells collectively ensure consistent lineage composition in the mouse blastocyst
Abstract:	Intercellular communication is essential to coordinate the behaviour of individual cells during organismal development. The preimplantation mammalian embryo is a paradigm of tissue self-organization and regulative development; however, the cellular basis of these regulative abilities has not been established. Here we use a quantitative image analysis pipeline to undertake a high-resolution, single-cell level analysis of lineage specification in the inner cell mass (ICM) of the mouse blastocyst. We show that a consistent ratio of epiblast and primitive endoderm lineages is achieved through incremental allocation of cells from a common progenitor pool, and that the lineage composition of the ICM is conserved regardless of its size. Furthermore, timed modulation of the FGF-MAPK pathway shows that individual progenitors commit to either fate asynchronously during blastocyst development. These data indicate that such incremental lineage allocation provides the basis for a tissue size control mechanism that ensures the generation of lineages of appropriate size.
Keywords:	self-renewal; expression; embryonic stem-cells; epiblast; primitive endoderm; nanog; inner; gata6; size regulation; mass cells
Journal Title:	Nature Communications
Volume:	7
ISSN:	2041-1723
Publisher:	Nature Publishing Group
Date Published:	2016-11-18
Start Page:	13463
Language:	English
ACCESSION:	WOS:000387993300001
DOI:	10.1038/ncomms13463
PROVIDER:	wos
PUBMED:	27857135
PMCID:	PMC5120222
Notes:	Article -- 13463 -- Source: Wos

https://synapse.mskcc.org/synapse/works/has_related_data_chk/103235