| Abstract: |
Mice carrying mutations at the W locus located on chromosome 5 are characterized by severe macrocytic anaemia, lack of hair pigmentation and sterility1. Mutations at this locus appear to affect the proliferation and/or migration of cells during early embryogenesis and result in an intrinsic defect in the haematopoietic stem cell hierarchy1,2. An understanding of the molecular basis of the complex and pleiotropic phenotype in W mutant mice would thus provide insights into the important developmental processes of gametogenesis, melanogenesis and haematopoiesis. Here we show that the mouse mutant W19H has a deletion of the c-kit proto-oncogene. Interspecific backcross analysis demonstrates that the W locus is very tightly linked to c-kit and that the two loci cannot be segregated at this level of analysis, c-kit is the cellular homologue of the oncogene v-kit of the HZ4 feline sarcoma virus3 and encodes a transmembrane protein tyrosine kinase receptor that is structurally similar to the receptors for colony-stimulating factor-1 (CSF-1) and platelet derived growth factor4,5. The co-localization of c-kit with W provides a molecular entry into this important region of the mouse genome. In addition, these observations provide the first example of a germ-line mutation in a mammalian proto-oncogene and implicate the c-kit gene as a candidate for the W locus. © 1988 Nature Publishing Group. |
| Keywords: |
mutation; proto-oncogene proteins; nonhuman; letter; mouse; animal; mice; gene; proto oncogene; mice, mutant strains; membrane proteins; protein tyrosine kinase; genetic engineering; hematopoiesis; melanogenesis; chromosome mapping; receptors, cell surface; proto-oncogenes; gametogenesis; locus; protein-tyrosine kinase; priority journal; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; proto-oncogene protein c-kit; enzyme receptor
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