| Abstract: |
Tumor acidosis and energy deprivation enhance thermal sensitivity. We have used in vivo 31 P nuclear magnetic resonance spectroscopy to noninvasively monitor changes in pH and energy metabolism in FSaII mouse tumors after i.p. administration of glucose. A dose of 5 g/kg glucose induced a pH drop of 0.31 units without any statistically significant change in energy status. The pH changes resolved within 2 h. In contrast, administration of 10 g/kg glucose resulted in a severe acidosis (mean nadir pH of 6.19 corresponding to a mean pH drop of 0.96 units) and loss of energy, the latter most probably being due to an acidosis-induced inhibition of glycolysis during ischemic hypoxia. The resulting acidosis and energy loss were more persistent and resolved in 5.5–28 h. In contrast, after an identical dose of mannitol (10 g/kg), a pH drop of approximately only 0.1 units over 72 min was noted. The data suggest that both cleavage of glucose to lactic acid and blood flow inhibition are involved in glucose induced tumor acidosis. In vivo 31 P nuclear magnetic resonance spectroscopy may be useful clinically to monitor therapeutic attempts at enhancing thermal sensitivity. © 1988, American Association for Cancer Research. All rights reserved. |
| Keywords: |
nonhuman; mouse; animal; metabolism; mice; animal experiment; dose-response relationship, drug; fibrosarcoma; magnetic resonance spectroscopy; glucose; energy metabolism; mannitol; nuclear magnetic resonance; hydrogen-ion concentration; mice, inbred c3h; heat; acidosis; priority journal; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; tumor bearing animal
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