| Abstract: |
The gene for human adenosine deaminase (ADA), an enzyme constitutively expressed in all tissues investigated so far and deficient in some cases of severe combined immune deficiency. was previously assigned to chromosome 20 by syntenic analysis. using somatic cell hybrids and quantitative enzyme studies on patients with chromosome abnormalities. Attempts at regional localization of ADA through indirect approaches have so far resulted in uncertainties, as well as apparent inconsistencies. In situhybridization of high-resolution somatic and pachytene chromosomes using a 3H-labeled cDNA probe of the ADA gene localized the gene to 20q 12→q 13.11. Rearrangements involving this region have been reported in various human hematological malignancies; in this regard, possible implications of the physical proximity of the ADA gene locus to that of SRC. an oncogene previously localized to the same region of chromosome 20, are briefly discussed. © 1989 S. Karger AG, Basel. |
| Keywords: |
human cell; pachytene; cytology; heredity; in situ hybridization; gene mapping; genetic engineering; nucleic acid hybridization; gene location; normal human; chromosome 20q; chromosome mapping; chromosomes, human, pair 20; adenosine deaminase; normal value; human; priority journal; nucleoside deaminases; support, non-u.s. gov't; support, u.s. gov't, p.h.s.
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