The cellular homologue of the transforming gene of SKV avian retrovirus maps to human chromosome region 1q22→q24 Journal Article
| Authors: | Chaganti, R. S. K.; Balazs, I.; Jhanwar, S. C.; Murty, V. V.; Koduru, P. R.; Grzeschik, K. H.; Stavnezer, E. |
| Article Title: | The cellular homologue of the transforming gene of SKV avian retrovirus maps to human chromosome region 1q22→q24 |
| Abstract: | We report the chromosomal localization of the cellular oncogene SKI, the putative oncogene of the Sloan-Kettering viruses (SKVs), a group of transforming retroviruses that had been isolated from chicken embryo cells infected with the avian leukosis virus tdB77. Southern blot analysis of DNA from mouse x human somatic cell hybrids with the v-SKI probe established synteny with chromosome 1, but excluding the region 1pter→q21. In situ hybridization of the same probe both to human spermatocyte pachytene and lymphocyte metaphase chromosomes enabled precise localization of the gene to the region 1q22→q24, a region that frequently is involved in translocations and other rearrangements in diverse human tumor types. In situ hybridization studies of metaphase spreads from a small noncleaved cell lymphoma that exhibited a t(1;14)(q21;q32) translocation showed that SKI translocates to the der(14) chromosome. Cytogenetic analysis of 65 prospectively ascertained non-Hodgkin's lymphomas revealed that the SKI region undergoes nonrandom breakage leading to translocations. Further analysis of the chromosome breaks in this group of lymphomas suggested that those involving the SKI site probably are of importance in tumor progression. |
| Keywords: | case report; cytology; animals; mice; heredity; avian leukosis virus; lymphatic system; in situ hybridization; cloning, molecular; aves; gene mapping; oncogene; nonhodgkin lymphoma; genetic engineering; recombination, genetic; radioisotope; chromosomes, human, pair 1; karyotyping; chromosome 1q; genetic markers; lymphocytoma; retrovirus; cell transformation, viral; chromosome mapping; chick embryo; southern blotting; proto-oncogenes; hybrid cell; hybrid cells; chromosome banding; genes, viral; unidentified retrovirus; humans; human; gallus gallus; chromosome translocation 1; avian retrovirus; gene assignment; leukosis virus |
| Journal Title: | Cytogenetics and Cell Genetics |
| Volume: | 43 |
| Issue: | 3-4 |
| ISSN: | 0301-0171 |
| Publisher: | S. Karger AG |
| Date Published: | 1986-01-01 |
| Start Page: | 181 |
| End Page: | 186 |
| Language: | English |
| PUBMED: | 3026737 |
| PROVIDER: | scopus |
| DOI: | 10.1159/000132318 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 18 August 2021 -- Source: Scopus |
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