Establishment of a human cell line (SKI-DLCL-1) with a t(1;14)(q21;q32) translocation from the ascites of a patient with diffuse large cell lymphoma Journal Article


Authors: Goy, A.; Gilles, F.; Remache, Y.; Filippa, D.; Portlock, C. S.; Jhanwar, S. C.; Zelenetz, A. D.
Article Title: Establishment of a human cell line (SKI-DLCL-1) with a t(1;14)(q21;q32) translocation from the ascites of a patient with diffuse large cell lymphoma
Abstract: Cytogenetic abnormalities at chromosome 1q21 are among the most common second genetic events observed in Non-Hodgkin's Lymphomas and have prognostic significance. Recently, BCL9 has been cloned from a pre-B-cell lymphoblastic leukemia cell line, which carried a t(1;14)(q21;q32). However, among a panel of 39 B-cell malignancies with 1q21 translocation, only two cases showed rearrangement for the BCL9 gene. We report the establishment of a new lymphoma cell line from a patient with relapsed diffuse large cell lymphoma. This cell line SKI-DLCL-1 showed cell surface antigens identical to the original tumor and demonstrated the profile of a mature B-cell phenotype: CD19 and CD20 positive, CD5 and C10 negative. It carried a t(1;14)(q21;q32) translocation identical to the original tumor. Although the clinical presentation was an isolated effusion lymphoma, studies for HIV-1, HHV8 and EBV were all negative. Southern blot analysis demonstrated that BCL9 was not rearranged in the SKI-DLCL-1 cell line. In addition, the BCL9 gene was not over-expressed in SKI-DLCL-1 cell line. In addition, the BCL9 gene was not over-expressed in SKI-DLCL-1 cell line. The identification of a new locus at 1q21 will help clarify the pathogenesis of B-cell malignancies with a translocation involving this locus.
Keywords: aged; unclassified drug; gene translocation; human cell; clinical feature; pathogenesis; cancer recurrence; case report; doxorubicin; ascites; phenotype; gene overexpression; carboplatin; cell maturation; etoposide; protein; gene locus; genetic association; cytogenetics; cyclophosphamide; vincristine; cancer cell culture; tumor cells, cultured; ifosfamide; procarbazine; b lymphocyte; chromosome aberration; prednisolone; lymphoma, b-cell; cd20 antigen; membrane antigen; nonhodgkin lymphoma; gene rearrangement; lymphoma; translocation, genetic; lymphoproliferative disease; large cell lymphoma; lymphatic leukemia; chromosomes, human, pair 1; cd5 antigen; cd19 antigen; epstein barr virus; cytogenetic analysis; human herpesvirus 8; human immunodeficiency virus 1; pre b lymphocyte; ascites fluid; leukemia cell line; common acute lymphoblastic leukemia antigen; translocation; southern blotting; cell cloning; lymphoma cell line; lymphoma, large-cell, diffuse; chromosomes, human, pair 14; chromosome translocation 14; humans; prognosis; human; male; priority journal; article; chromosome translocation 1; iq21; t(1; 14)(q21; q32); protein bcl 9
Journal Title: Leukemia and Lymphoma
Volume: 40
Issue: 3-4
ISSN: 1042-8194
Publisher: Taylor & Francis Group  
Date Published: 2001-01-01
Start Page: 419
End Page: 423
Language: English
PUBMED: 11426565
PROVIDER: scopus
DOI: 10.3109/10428190109057942
DOI/URL:
Notes: Export Date: 21 May 2015 -- Source: Scopus
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MSK Authors
  1. Carol Portlock
    181 Portlock
  2. Frederic Gilles
    19 Gilles
  3. Andrew D Zelenetz
    556 Zelenetz
  4. Daniel A Filippa
    102 Filippa
  5. Suresh C Jhanwar
    217 Jhanwar
  6. Andre Goy
    28 Goy
  7. Yvonne K Remache
    13 Remache