Deregulation of FCGR2B expression by 1q21 rearrangements in follicular lymphomas Journal Article

Authors: Chen, W.; Palanisamy, N.; Schmidt, H.; Teruya-Feldstein, J.; Jhanwar, S. C.; Zelenetz, A. D.; Houldsworth, J.; Chaganti, R. S. K.
Article Title: Deregulation of FCGR2B expression by 1q21 rearrangements in follicular lymphomas
Abstract: We report here the molecular cloning and characterization of a t(1;14)(q21;q32) in a follicular lymphoma (FL) with an unusual BCL2 aberration. Fluorescence in situ hybridization (FISH) and Southern blot analysis of tumor cells identified the translocation breakpoint within the 5′ switch region of IGHG (Sγ). We cloned the chimeric breakpoint region approximately 1.5 kbp downstream from the HindIII site of 5′Sγ2 on chromosome 14q32 and identified a 360-bp novel segment with homology to the CpG island clone 11h8. Two BAC clones containing this sequence were isolated and mapped to 1q21 by FISH. BAC 342/P13 contained sequences homologous to Fcγ receptors 2A, 3A, 2B, 3B, and a heat shock protein gene HSP70B. The translocation brought the Sγ2 region of a productive IGH allele 20∼30 kbp upstream of FCGR2B. As a result of the translocation, the b2 isoform of FCGR2B was overexpressed in the tumor. Screening of a panel of 76 B-cell lymphomas with 1q21-23 cytogenetic aberrations by Southern blot analysis using breakpoint probes identified an additional FL with a t(14;18)(q32;q21) and a breakpoint in the FCGR2B region. These results suggest that FCGR2B may be deregulated by 1q21 aberration in BCL2 rearranged FLs and possibly play a role in their progression.
Keywords: immunohistochemistry; adult; human tissue; human cell; case report; allele; in situ hybridization, fluorescence; gene overexpression; protein bcl 2; reverse transcription polymerase chain reaction; gene expression; gene expression regulation; cloning, molecular; fluorescence in situ hybridization; gene rearrangement; lymphoma, non-hodgkin; cpg island; chromosome rearrangement; translocation, genetic; fc receptor; receptors, igg; heat shock protein 70; antigens, cd; chromosome aberrations; sequence homology; chromosomes, human, pair 1; follicular lymphoma; proto-oncogene proteins c-bcl-2; lymphoma, follicular; chromosome 1q; metaphase; mutagenesis, insertional; chromosome 14q; chromosome map; translocation; southern blotting; chromosomes, human, pair 14; blotting, southern; humans; human; female; priority journal; article; staphylococcus phage 3a; chromosome translocation 1; 1q21; fcgr2b
Journal Title: Oncogene
Volume: 20
Issue: 52
ISSN: 0950-9232
Publisher: Nature Publishing Group  
Date Published: 2001-11-15
Start Page: 7686
End Page: 7693
Language: English
DOI: 10.1038/sj/onc/1204989
PUBMED: 11753646
PROVIDER: scopus
Notes: Export Date: 21 May 2015 -- Source: Scopus
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