Cytogenetic and molecular analysis of 6q deletions in Burkitt's lymphoma cell lines Journal Article
| Authors: | Parsa, N. Z.; Gaidano, G.; Mukherjee, A. B.; Hauptschein, R. S.; Lenoir, G.; Dalla‐Favera, R.; Chaganti, R. S. K. |
| Article Title: | Cytogenetic and molecular analysis of 6q deletions in Burkitt's lymphoma cell lines |
| Abstract: | Although abnormalities of chromosome 6 have frequently been observed in Burkitt's lymphoma (BL), they have 50 far not been defined by modern cytogenetic and molecular methods. By a combination of high‐resolution chromosome banding, fluorescence in situ hybridization (FISH), and loss of heterozygosity (LOH) analysis, we have examined the nature of aberrations affecting chromosome 6 in 7 previously established BL cell lines. All cell lines exhibited the characteristic translocations associated with BL; 5 had t(814)(q24;q32) and 2 had t(8;22)(q24;q11). Three cell lines had deletions of 6q; 3 others had rearrangements affecting 6q, whereas one cell line had apparently normal chromosomes 6. FISH analysis of the three deletions established that they were interstitial. LOH analysis with probes mapped to the 6q26‐27 region confirmed the sub‐telomeric interstitial deletion in cell line BL‐108, which had a del(6)(q23q27). All informative loci mapped to 6q26‐27 (5/7) were deleted in BL‐74, which had no apparent cytogenetic abnormality in chromosome 6, thus documenting a sub‐microscopic deletion. These data define the cytogenetic and molecular limits of 6q deletions in BL and are consistent with our previous demonstration of LOH analysis of the site of a candidate tumor suppressor gene in the 6q25‐27 region. Genes Chrom Cancer 9:13‐18 (1994). ©1994 Wiley‐Liss, Inc. Copyright © 1994 Wiley‐Liss, Inc., A Wiley Company |
| Keywords: | adolescent; child; human cell; in situ hybridization, fluorescence; gene locus; cytogenetics; cancer cell culture; tumor cells, cultured; tumor suppressor gene; dna, neoplasm; chromosomes, human, pair 6; chromosome deletion; burkitt lymphoma; chromosome 6q; chromosomes, human, pair 22; chromosome translocation 8; chromosome banding; chromosomes, human, pair 14; human; male; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s. |
| Journal Title: | Genes Chromosomes and Cancer |
| Volume: | 9 |
| Issue: | 1 |
| ISSN: | 1045-2257 |
| Publisher: | Wiley Periodicals, Inc |
| Date Published: | 1994-01-01 |
| Start Page: | 13 |
| End Page: | 18 |
| Language: | English |
| DOI: | 10.1002/gcc.2870090104 |
| PROVIDER: | scopus |
| PUBMED: | 7507695 |
| DOI/URL: | |
| Notes: | Export Date: 14 January 2019 -- Article -- Source: Scopus |
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