Biological applications of hyperpolarized (13)C NMR Book Section
| Authors: | Keshari, K. R.; Tikunov, A. P.; Lee, H.; Macdonald, J. M. |
| Editors: | Lindon, J. C.; Tranter, G. E.; Koppenaal, D. W. |
| Article/Chapter Title: | Biological applications of hyperpolarized (13)C NMR |
| Abstract: | 13C NMR spectroscopy of biological systems, including living systems and extracted material, has been limited due to its relatively low sensitivity, associated with its low gyromagnetic ratio. Newer methods of hyperpolarization including dynamic nuclear polarization (DNP) and para-hydrogen-induced polarization (PHIP) can increase sensitivity by nearly five orders of magnitude, thus permitting 13C magnetic resonance images (MRI) as sensitive as obtained by 1H MRI based on 1H2O. DNP is based on the polarization of nuclear spins in an amorphous solid state at ~ 1 K through the coupling of the nuclear spins and unpaired electrons via an organic-free radical. PHIP is a chemistry-based technique and relies on the introduction of a para-hydrogen substrate through catalytic hydrogenation. This article describes and gives a historical perspective and the physics, hardware, and NMR pulse sequences used in DNP and in PHIP applications while giving an example of measuring flux by DNP using an NMR-compatible bioreactor system. © 2017 Elsevier Ltd. unless otherwise stated. All rights reserved. |
| Keywords: | inept; polarization transfer; dynamic nuclear polarization (dnp); 13c flux; 13c mri; optical pumping; para-hydrogen-induced polarization (phip); solid-state effect |
| Book Title: | Encyclopedia of Spectroscopy and Spectrometry. 3rd ed |
| ISBN: | 978-0-12-803224-4 |
| Publisher: | Elsevier Inc. |
| Publication Place: | Oxford, United Kingdom |
| Date Published: | 2017-01-01 |
| Start Page: | 143 |
| End Page: | 148 |
| Language: | English |
| DOI: | 10.1016/b978-0-12-409547-2.12116-x |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Book Chapter -- Export Date: 2 March 2020 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
MSK Authors
Related MSK Work