Rapid confocal imaging of large areas of excised tissue with strip mosaicing Journal Article
| Authors: | Abeytunge, S.; Li, Y.; Larson, B.; Toledo-Crow, R.; Rajadhyaksha, M. |
| Article Title: | Rapid confocal imaging of large areas of excised tissue with strip mosaicing |
| Abstract: | Imaging large areas of tissue rapidly and with high resolution may enable rapid pathology at the bedside. The limited field of view of high-resolutionmicroscopes requires themerging of multiple images that are taken sequentially to cover a large area. This merging or mosaicing of images requires long acquisition and processing times, and produces artifacts. To reduce both time and artifacts, we developed a mosaicing method on a confocal microscope that images morphology in large areas of excised tissuewith sub-cellular detail. By acquiring image strips with aspect ratios of 10:1 and higher (instead of the standard ∼1:1) and "stitching" them in software, our method images 10×10 mm2 area of tissue in about 3 min. This method, which we call "strip mosaicing," is currently three times as fast as our previous method. © 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). |
| Keywords: | basal cell carcinoma; pathology; surgery; mohs surgery; surgical pathology; diseases; tissue; confocal fluorescence mosaicing microscopy; large area imaging microscopy; aspect ratio |
| Journal Title: | Journal of Biomedical Optics |
| Volume: | 16 |
| Issue: | 5 |
| ISSN: | 1083-3668 |
| Publisher: | SPIE |
| Date Published: | 2011-01-01 |
| Start Page: | 050504 |
| Language: | English |
| DOI: | 10.1117/1.3582335 |
| PROVIDER: | scopus |
| PMCID: | PMC3117897 |
| PUBMED: | 21639560 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 9 December 2011" - "CODEN: JBOPF" - "Source: Scopus" |
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222Rajadhyaksha -
25Toledo-Crow -
11Larson -
32Abeytunge -
20Li
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