Confocal microscopy with strip mosaicing for rapid imaging over large areas of excised tissue Journal Article

   


Authors: Abeytunge, S.; Li, Y.; Larson, B.; Peterson, G.; Seltzer, E.; Toledo-Crow, R.; Rajadhyaksha, M.
Article Title: Confocal microscopy with strip mosaicing for rapid imaging over large areas of excised tissue
Abstract: Confocal mosaicing microscopy is a developing technology platform for imaging tumor margins directly in freshly excised tissue, without the processing required for conventional pathology. Previously, mosaicing on 12-×-12 mm2 of excised skin tissue from Mohs surgery and detection of basal cell carcinoma margins was demonstrated in 9 min. Last year, we reported the feasibility of a faster approach called "strip mosaicing," which was demonstrated on a 10-×-10 mm2 of tissue in 3 min. Here we describe further advances in instrumentation, software, and speed. A mechanism was also developed to flatten tissue in order to enable consistent and repeatable acquisition of images over large areas. We demonstrate mosaicing on 10-×-10 mm2 of skin tissue with 1-μm lateral resolution in 90 s. A 2.5-×-3.5 cm2 piece of breast tissue was scanned with 0.8-μm lateral resolution in 13 min. Rapid mosaicing of confocal images on large areas of fresh tissue potentially offers a means to perform pathology at the bedside. Imaging of tumor margins with strip mosaicing confocal microscopy may serve as an adjunct to conventional (frozen or fixed) pathology for guiding surgery.
Keywords: methodology; statistics; basal cell carcinoma; confocal microscopy; microscopy, confocal; skin neoplasms; pathology; breast neoplasms; algorithms; histology; histological techniques; skin tumor; algorithm; evaluation; breast tumor; equipment design; carcinoma, basal cell; mohs surgery; chemosurgery; pathology, surgical; equipment; optical phenomena; light related phenomena
Journal Title: Journal of Biomedical Optics
Volume: 18
Issue: 6
ISSN: 1083-3668
Publisher: SPIE  
Date Published: 2013-01-01
Start Page: 61227
Language: English
PUBMED: 23389736
PROVIDER: scopus
PMCID: PMC3565124
DOI: 10.1117/1.jbo.18.6.061227
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-84878482144&partnerID=40&md5=8bc4195b05fb7f387e9eceec31a5c3f8
Notes: --- - Cited By (since 1996):1 - "Export Date: 1 July 2013" - "Source: Scopus"
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MSK Authors
  1. Milind Rajadhyaksha
    225 Rajadhyaksha
  2. Ricardo Toledo-Crow
    25 Toledo-Crow
  3. Bjorg Larson
    11 Larson
  4. Sanjeewa Abeytunge
    32 Abeytunge
  5. Gary Peterson
    30 Peterson
  6. Yongbiao Li
    20 Li
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