Molecular basis for control of diverse genome stability factors by the multi-BRCT scaffold Rtt107 Journal Article

Authors: Wan, B.; Wu, J.; Meng, X.; Lei, M.; Zhao, X.
Article Title: Molecular basis for control of diverse genome stability factors by the multi-BRCT scaffold Rtt107
Abstract: BRCT domains support myriad protein-protein interactions involved in genome maintenance. Although di-BRCT recognition of phospho-proteins is well known to support the genotoxic response, whether multi-BRCT domains can acquire distinct structures and functions is unclear. Here we present the tetra-BRCT structures from the conserved yeast protein Rtt107 in free and ligand-bound forms. The four BRCT repeats fold into a tetrahedral structure that recognizes unmodified ligands using a bi-partite mechanism, suggesting repeat origami enabling function acquisition. Functional studies show that Rtt107 binding of partner proteins of diverse activities promotes genome replication and stability in both distinct and concerted manners. A unified theme is that tetra- and di-BRCT domains of Rtt107 collaborate to recruit partner proteins to chromatin. Our work thus illustrates how a master regulator uses two types of BRCT domains to recognize distinct genome factors and direct them to chromatin for constitutive genome protection. © 2019 Elsevier Inc. Di-BRCT domains recognize phospho-proteins and contribute to the DNA damage response. Wan et al. show that a distinct architecture formed by four BRCTs of the Rtt107 protein enables recognition of phospho-free ligands and supports constitutive genome protection. This work uncovers structural plasticity and functional diversity of BRCT domains. © 2019 Elsevier Inc.
Keywords: unclassified drug; protein domain; protein protein interaction; chromatin; genomic instability; molecular recognition; ligand; protein structure; phosphoprotein; genome maintenance; protein interaction networks; smc5/6; article; molecular scaffold; mms22; rtt107; rtt107 protein; di-brct domains; rdna stability; slx4; synthetic interactions; tetra-brct domains
Journal Title: Molecular Cell
Volume: 75
Issue: 2
ISSN: 1097-2765
Publisher: Cell Press  
Date Published: 2019-07-25
Start Page: 238
End Page: 251.e5
Language: English
DOI: 10.1016/j.molcel.2019.05.035
PUBMED: 31348879
PROVIDER: scopus
PMCID: PMC6745058
Notes: Article -- Export Date: 4 September 2019 -- Source: Scopus
Citation Impact
MSK Authors
  1. Xiaolan Zhao
    55 Zhao
  2. Bingbing   Wan
    3 Wan
  3. Xiangzhou Meng
    4 Meng