Crystal structure of human 53BP1 BRCT domains bound to p53 tumour suppressor Journal Article

Authors: Derbyshire, D. J.; Basu, B. P.; Serpell, L. C.; Joo, W. S.; Date, T.; Iwabuchi, K.; Doherty, A. J.
Article Title: Crystal structure of human 53BP1 BRCT domains bound to p53 tumour suppressor
Abstract: The BRCT (BRCA1 C-terminus) is an evolutionary conserved protein-protein interacting module found as single, tandem or multiple repeats in a diverse range of proteins known to play roles in the DNA-damage response. The BRCT domains of 53BP1 bind to the tumour suppressor p53. To investigate the nature of this interaction, we have determined the crystal structure of the 53BP1 BRCT tandem repeat in complex with the DNA-binding domain of p53. The structure of the 53BP1-p53 complex shows that the BRCT tandem repeats pack together through a conserved interface that also involves the inter-domain linker. A comparison of the structure of the BRCT region of 53BP1 with the BRCA1 BRCT tandem repeat reveals that the interdomain interface and linker regions are remarkably well conserved. 53BP1 binds to p53 through contacts with the N-terminal BRCT repeat and the inter-BRCT linker. The p53 residues involved in this binding are mutated in cancer and are also important for DNA binding. We propose that BRCT domains bind to cellular target proteins through a conserved structural element termed the 'BRCT recognition motif'.
Keywords: dna repair; protein; transcriptional activation; brca1; p53; damage; binding; complex; iv; strand breaks; interacts; wild-type; 53bp1; brct; dna-ligase-iii
Journal Title: EMBO Journal
Volume: 21
Issue: 14
ISSN: 0261-4189
Publisher: Wiley Blackwell  
Date Published: 2002-07-15
Start Page: 3863
End Page: 3872
Language: English
ACCESSION: WOS:000176784700029
DOI: 10.1093/emboj/cdf383
PMCID: PMC126127
PUBMED: 12110597
Notes: Article -- Source: Wos
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MSK Authors
  1. Woo S Joo
    4 Joo