Rtt107 is a multi-functional scaffold supporting replication progression with partner SUMO and ubiquitin ligases Journal Article


Authors: Hang, L. E.; Peng, J.; Tan, W.; Szakal, B.; Menolfi, D.; Sheng, Z.; Lobachev, K.; Branzei, D.; Feng, W.; Zhao, X.
Article Title: Rtt107 is a multi-functional scaffold supporting replication progression with partner SUMO and ubiquitin ligases
Abstract: Elucidating the individual and collaborative functions of genome maintenance factors is critical for understanding how genome duplication is achieved. Here, we investigate a conserved scaffold in budding yeast, Rtt107, and its three partners: a SUMO E3 complex, a ubiquitin E3 complex, and Slx4. Biochemical and genetic findings show that Rtt107 interacts separately with these partners and contributes to their individual functions, including a role in replisome sumoylation. We also provide evidence that Rtt107 associates with replisome components, and both itself and its associated E3s are important for replicating regions far from initiation sites. Corroborating these results, replication defects due to Rtt107 loss and genotoxic sensitivities in mutants of Rtt107 and its associated E3s are rescued by increasing replication initiation events through mutating two master repressors of late origins, Mrc1 and Mec1. These findings suggest that Rtt107 functions as a multi-functional platform to support replication progression with its partner E3 enzymes. © 2015 Elsevier Inc.
Journal Title: Molecular Cell
Volume: 60
Issue: 2
ISSN: 1097-2765
Publisher: Cell Press  
Date Published: 2015-10-15
Start Page: 268
End Page: 279
Language: English
DOI: 10.1016/j.molcel.2015.08.023
PROVIDER: scopus
PMCID: PMC4609303
PUBMED: 26439300
DOI/URL:
Notes: Export Date: 2 November 2015 -- Source: Scopus
Altmetric
Citation Impact
MSK Authors
  1. Xiaolan Zhao
    55 Zhao
  2. Lisa Eileen Hang
    9 Hang
  3. Wei   Tan
    1 Tan