Application of DNA amplification to pneumocystosis: Presence of serum Pneumocystis carinii dna during human and experimentally induced Pneumocystis carinii pneumonia Journal Article


Authors: Schluger, N.; Godwin, T.; Sepkowitz, K.; Armstrong, D.; Bernard, E.; Rifkin, M.; Cerami, A.; Bucala, R.
Article Title: Application of DNA amplification to pneumocystosis: Presence of serum Pneumocystis carinii dna during human and experimentally induced Pneumocystis carinii pneumonia
Abstract: Pneumocystis carinii pneumonia is a leading cause of morbidity and mortality in patients with the acquired immunodeficiency syndrome (AIDS). Much remains unknown about the basic biology ofP. carinii and studies of this infection have been hampered by the lack of cultivation methods. We developed a sensitive and specific assay for P. carinii by utilizing DNA amplification of the P. carinii dihydrofolate reductase (DHFR) gene. By this method, P. carinii DNA was detected in the lungs of rats with experimentally induced P. carinii pneumonia 2 wk before the onset of histopathological changes. DNA amplification analysis of serum demonstrated that by 10 wk ofcorticosteroid treatment, 12 of 12 (100%) infected rats had circulating DHFR DNA. P. carinii DHFR DNA also was detected in the serum ofpatients with AIDS and active P. carinii pneumonia (12 of 14 sera collected prospectively). Patients with advanced AIDS but without a history of P. carinii pneumonia were negative by this assay (0 of 6 sera examined). Serum polymerase chain reaction may facilitate investigations into the natural history and epidemiology ofP. carinii infection, provide insight into the pathogenesis of parasite dissemination, and offer a useful, noninvasive diagnostic test for the detection of human pneumocystosis. © 1992, Rockefeller University Press., All rights reserved.
Keywords: controlled study; nonhuman; polymerase chain reaction; animal; animal tissue; gene amplification; animal experiment; pneumocystis pneumonia; dna; molecular sequence data; rat; base sequence; rats; acquired immune deficiency syndrome; acquired immunodeficiency syndrome; dihydrofolate reductase; tetrahydrofolate dehydrogenase; serum; dna determination; dna, fungal; lung parenchyma; pneumocystosis; pneumocystis carinii; human; female; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; pneumonia, pneumocystis carinii
Journal Title: Journal of Experimental Medicine
Volume: 176
Issue: 5
ISSN: 0022-1007
Publisher: Rockefeller University Press  
Date Published: 1992-11-01
Start Page: 1327
End Page: 1333
Language: English
DOI: 10.1084/jem.176.5.1327
PUBMED: 1402679
PROVIDER: scopus
PMCID: PMC2119432
DOI/URL:
Notes: Article -- Source: Scopus
Altmetric
Citation Impact
MSK Authors
  1. Kent A Sepkowitz
    270 Sepkowitz
  2. Donald Armstrong
    224 Armstrong
  3. Edward M Bernard
    49 Bernard