DNA amplification in experimental pneumocystosis: Characterization of serum Pneumocystis carinii DNA and potential P. carinii carrier states Journal Article

  

Authors:	Sepkowitz, K.; Schluger, N.; Godwin, T.; Armstrong, D.; Cerami, A.; Bucala, R.
Article Title:	DNA amplification in experimental pneumocystosis: Characterization of serum Pneumocystis carinii DNA and potential P. carinii carrier states
Abstract:	DNA amplification has identified P. carinii DNA in diverse biologic specimens, including the serum of patients with P. carinii pneumonia. To further examine the relationship between P. carinii DNA in serum and P. carinii infection, the corticosteroid-treated rat model of pneumocystosis was studied. By 4 weeks ofimmunosuppression, P. carinii DNA was detected in rat lungs and by 6 weeks, in their serum. P. carinii DNA persisted in lung tissue as long as 5 months after the withdrawal of steroids. Serum DNA disappeared 2 weeks after steroids were withdrawn. Nonimmunocompromised, sentinel rats housed near immunocompromised, P. carin;;-infected rats also were studied. Within 6 weeks, P. carin;;DNA became detectable in lung and by 8 weeks, in serum. P. cariniiDNA disappeared rapidly from lungs and sera after sentinel rats were isolated away from corticosteroid-treated rats. These findings support the contagious transmission of P. carinii and suggest facile development of P. carinii carrier states. © 1993 by The University of Chicago.
Keywords:	nonhuman; prospective study; polymerase chain reaction; animal; animal tissue; gene amplification; animal experiment; animal model; molecular sequence data; rat; lung; base sequence; rats; rats, sprague-dawley; immune deficiency; immunosuppressive treatment; dihydrofolate reductase; disease transmission; opportunistic infection; dna, fungal; dna template; lung homogenate; pneumocystosis; pneumocystis carinii; disease carrier; female; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; pneumonia, pneumocystis carinii
Journal Title:	Journal of Infectious Diseases
Volume:	168
Issue:	2
ISSN:	0022-1899
Publisher:	Oxford University Press
Date Published:	1993-08-01
Start Page:	421
End Page:	426
Language:	English
DOI:	10.1093/infdis/168.2.421
PUBMED:	8335980
PROVIDER:	scopus
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027275378&doi=10.1093%2finfdis%2f168.2.421&partnerID=40&md5=f41d913f9a094b1660d774ce38f218c6
Notes:	Article -- Export Date: 1 March 2019 -- Source: Scopus

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