Expression of disialogangliosides GD2 and GD3 on human soft tissue sarcomas Journal Article

  


Authors: Chang, H. R.; Cordon-Cardo, C.; Houghton, A. N.; Cheung, N. V.; Brennan, M. F.
Article Title: Expression of disialogangliosides GD2 and GD3 on human soft tissue sarcomas
Abstract: Methods. Fifty‐six freshly frozen human sarcomas were studied for expression of disialogangliosides by avi‐din‐biotin immunohistochemical staining with purified monoclonal antibodies (MoAb) 3F8 (antidisialoganglioside GD2) and R24 (antidisialoganglioside GD3). Results. Ninety‐three percent of the tumors tested by the immunohistochemical staining expressed GD2 and 88% expressed GD3. The intensity of expression varied among sarcomas of different histologic types. Lipo‐sarcoma, fibrosarcoma, malignant fibrous histiocytoma, leiomyosarcoma, and spindle cell sarcoma reacted strongly with 3F8 and R24. Embryonal rhabdomyosarcoma and synovial sarcoma demonstrated substantially weaker staining by either MoAb. Weakly reactive or nonreactive tumors were, in general, high‐grade or meta‐static sarcomas. Ganglioside extraction and thin‐layer chromatography/immunothin‐layer chromatography of two liposarcomas confirmed the identities of these gangliosides. Conclusions. GD2 and GD3 may indicate sites for MoAb‐targeted imaging and therapy for sarcomas. The association of a diminished stainability by 3F8 and R24 with aggressive sarcomas may indicate prognostic significance. Cancer 1992; 70:633–638. Copyright © 1992 American Cancer Society
Keywords: immunohistochemistry; human tissue; major clinical study; cancer localization; antigen expression; metastasis; immunoreactivity; histology; sarcoma; immunoenzyme techniques; fibrosarcoma; tumor antigens; soft tissue sarcoma; leiomyosarcoma; malignant fibrous histiocytoma; rhabdomyosarcoma; ganglioside gd3; soft tissue neoplasms; liposarcoma; gangliosides; gd2; prognosis; human; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; gd3; soft tissue sarcomas; disialogangliosides; histopathologies
Journal Title: Cancer
Volume: 70
Issue: 3
ISSN: 0008-543X
Publisher: Wiley Blackwell  
Date Published: 1992-08-01
Start Page: 633
End Page: 638
Language: English
DOI: 10.1002/1097-0142(19920801)70:3<633::Aid-cncr2820700315>3.0.Co;2-f
PUBMED: 1623478
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026690779&doi=10.1002%2f1097-0142%2819920801%2970%3a3%3c633%3a%3aAID-CNCR2820700315%3e3.0.CO%3b2-F&partnerID=40&md5=669af8cf4be6a6b8bc0675c34fc051be
Notes: Article -- Export Date: 30 July 2019 -- Source: Scopus
Altmetric
What is Altmetric?
Citation Impact
What is Dimensions Citation Badge?
BMJ Impact Analytics
MSK Authors
  1. Murray F Brennan
    1059 Brennan
  2. Nai-Kong Cheung
    648 Cheung
  3. Carlos Cordon-Cardo
    612 Cordon-Cardo
  4. Alan N Houghton
    364 Houghton
Related MSK Work