Effect of hyperinsulinemia on whole body and skeletal muscle leucine carbon kinetics in humans Journal Article
| Authors: | Heslin, M. J.; Newman, E.; Wolf, R. F.; Pisters, P. W. T.; Brennan, M. F. |
| Article Title: | Effect of hyperinsulinemia on whole body and skeletal muscle leucine carbon kinetics in humans |
| Abstract: | Data documenting the isolated effect of systemic hyperinsulinemia on whole body and skeletal muscle leucine carbon kinetics in humans are limited. Using steady-state [14C]leucine kinetics, 10 normal volunteers were studied in the baseline postabsorptive state and then under euglycemic, hyperinsulinemic (71 ± 5 μU/ml), and euleucinemic conditions. Systemic hyperinsulinemia resulted in a significant decrease in whole body and forearm leucine rate of appearance (R(a)) by 17 and 37%, respectively, (P < 0.0003, 0.03), without a significant change in the nonoxidized rate of disappearance for either (P = 0.23, 0.66). The baseline contribution of total body skeletal muscle (TBSM) leucine R(a) and rate of disappearance (R(d)) to whole body leucine R(a) and R(d) was 27 ± 6 and 24 ± 5%, respectively. During hyperinsulinemia TBSM R(a) decreased by 34%, whereas whole body R(a) decreased by 16%. We conclude that the primary effect of insulin in the whole body and skeletal muscle is to decrease leucine release from protein without a significant effect on leucine incorporation into protein. This antiproteolytic effect of insulin is more pronounced in skeletal muscle than in other tissues in the body. |
| Keywords: | models, biological; protein metabolism; protein; kinetics; protein synthesis; insulin; amino acid; glucose; amino acids; leucine; sodium; normal human; skeletal muscle; human experiment; muscles; plethysmography; middle age; hyperinsulinemia; hyperinsulinism; carbon radioisotopes; sodium bicarbonate; human; male; female; priority journal; article; radioisotope dilution technique; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; bicarbonates; human insulin; forearm blood flow |
| Journal Title: | American Journal of Physiology - Endocrinology and Metabolism |
| Volume: | 262 |
| Issue: | 6 |
| ISSN: | 0193-1849 |
| Publisher: | American Physiological Society |
| Date Published: | 1992-06-01 |
| Start Page: | E911 |
| End Page: | E918 |
| Language: | English |
| PUBMED: | 1319683 |
| PROVIDER: | scopus |
| DOI: | 10.1152/ajpendo.1992.262.6.E911 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 30 July 2019 -- Source: Scopus |
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