Growth hormone and insulin combine to improve whole-body and skeletal muscle protein kinetics Journal Article


Authors: Wolf, R. F.; Heslin, M. J.; Newman, E.; Pearlstone, D. B.; Gonenne, A.; Brennan, M. F.
Article Title: Growth hormone and insulin combine to improve whole-body and skeletal muscle protein kinetics
Abstract: Background. A cooperative effect of exogenous insulin and recombinant human growth hormone (r-hGH) with respect to whole-body and skeletal muscle protein metabolism has not been demonstrated previously. This study examined the effect of r-hGH and insulin administration during euglycemic clamping and concurrent amino acid supplementation. Methods. Twenty-three normal volunteers in the postabsorptive state were either treated with r-hGH for 3 consecutive days before a metabolic study (GH group; n = 10) or not treated (CTRL group; n = 13). The r-hGH dose was 0.2 mg/kg/day (n = 5) or 0.1 mg/kg/day (n = 5). All subjects then received an infusion of 14C-labeled leucine and tritiated phenylalanine, followed by measurement of baseline protein kinetics (GH and CTRL). Subsequently a euglycemic insulin infusion (1 mU/kg/min) with concurrent amino acid infusion was administered, and protein kinetic measurements were repeated at steady state. Results. GH and insulin separately produced an increase in whole-body and skeletal muscle protein net balance. GH plus insulin was associated with a higher net balance of protein than was insulin alone. Conclusions. r-hGH and insulin in the presence of amino acids and glucose combine to improve whole-body and skeletal muscle protein kinetics. © 1992.
Keywords: somatomedin; adult; controlled study; dose response; conference paper; proteins; protein metabolism; protein; dose-response relationship, drug; diet supplementation; growth hormone; drug synergism; kinetics; recombinant proteins; human growth hormone; insulin; glucose blood level; amino acid; glucose; blood glucose; amino acid blood level; amino acids; leucine; intravenous drug administration; normal human; skeletal muscle; phenylalanine; human experiment; regional blood flow; forearm; amino acid metabolism; seric gonadotropin; glucagon; muscle proteins; subcutaneous drug administration; osmolar concentration; human; male; female; priority journal; support, non-u.s. gov't; forearm blood flow
Journal Title: Surgery
Volume: 112
Issue: 2
ISSN: 0039-6060
Publisher: Elsevier Inc.  
Date Published: 1992-08-01
Start Page: 284
End Page: 291; discussion 291-292
Language: English
PUBMED: 1641767
PROVIDER: scopus
DOI/URL:
Notes: Source: Scopus
Citation Impact
MSK Authors
  1. Murray F Brennan
    860 Brennan
  2. Martin J. Heslin
    28 Heslin