The ubiquitous subunit of erythroid transcription factor NF-E2 is a small basic-leucine zipper protein related to the v-maf oncogene Journal Article
| Authors: | Andrews, N. C.; Kotkow, K. J.; Ney, P. A.; Erdjument-Bromage, H.; Tempst, P.; Orkin, S. H. |
| Article Title: | The ubiquitous subunit of erythroid transcription factor NF-E2 is a small basic-leucine zipper protein related to the v-maf oncogene |
| Abstract: | Erythroid transcription factor NF-E2 is a tissue-restricted heterodimeric protein which recognizes an extended AP-1 motif [(T/C)TGCTGA(C/G)TCA(T/C)] found in the upstream locus control regions of the alpha- and beta-globin gene clusters. A cDNA clone encoding a cell-type-specific subunit of NF-E2, designated p45 NF-E2, has previously been characterized and shown to encode a basic-leucine zipper DNA-binding protein. Here we describe protein purification and cloning of cDNA that encodes the second basic-leucine zipper subunit of the native NF-E2 heterodimer. This polypeptide, designated p18, is widely expressed. It displays extensive homology to the v-maf oncogene product and a human retinal-specific protein, NRL. Unusual features in the basic region shared by v-Maf, NRL, and p18 place them in a distinct subfamily of AP-1-like proteins. |
| Keywords: | gene; invivo; mammalian-cells; dna-binding protein; globin gene expression; locus control region; locus-control region; dominant control region; c-jun; porphobilinogen deaminase; beta-globin gene; regulatory element; ap-1 motif; jun-b |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 90 |
| Issue: | 24 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 1993-12-15 |
| Start Page: | 11488 |
| End Page: | 11492 |
| Language: | English |
| ACCESSION: | WOS:A1993MM51500012 |
| DOI: | 10.1073/pnas.90.24.11488 |
| PROVIDER: | wos |
| PMCID: | PMC48009 |
| PUBMED: | 8265578 |
| Notes: | Article -- Source: Wos |
