DNA-enabled rational design of fluorescence-Raman bimodal nanoprobes for cancer imaging and therapy Journal Article

   


Authors: Pal, S.; Ray, A.; Andreou, C.; Zhou, Y.; Rakshit, T.; Wlodarczyk, M.; Maeda, M.; Toledo-Crow, R.; Berisha, N.; Yang, J.; Hsu, H. T.; Oseledchyk, A.; Mondal, J.; Zou, S.; Kircher, M. F.
Article Title: DNA-enabled rational design of fluorescence-Raman bimodal nanoprobes for cancer imaging and therapy
Abstract: Recently, surface-enhanced Raman scattering nanoprobes have shown tremendous potential in oncological imaging owing to the high sensitivity and specificity of their fingerprint-like spectra. As current Raman scanners rely on a slow, point-by-point spectrum acquisition, there is an unmet need for faster imaging to cover a clinically relevant area in real-time. Herein, we report the rational design and optimization of fluorescence-Raman bimodal nanoparticles (FRNPs) that synergistically combine the specificity of Raman spectroscopy with the versatility and speed of fluorescence imaging. DNA-enabled molecular engineering allows the rational design of FRNPs with a detection limit as low as 5 × 10 −15 M. FRNPs selectively accumulate in tumor tissue mouse cancer models and enable real-time fluorescence imaging for tumor detection, resection, and subsequent Raman-based verification of clean margins. Furthermore, FRNPs enable highly efficient image-guided photothermal ablation of tumors, widening the scope of the NPs into the therapeutic realm. © 2019, The Author(s).
Keywords: controlled study; human cell; genetics; nonhuman; nuclear magnetic resonance imaging; brain tumor; brain neoplasms; sensitivity and specificity; ovarian neoplasms; mouse; animal; metabolism; animals; mice; cell viability; confocal microscopy; fluorescence; fluorescent dye; fluorescent dyes; animal experiment; animal model; immunofluorescence; drug screening; xenograft model antitumor assays; cell line, tumor; diagnostic imaging; cancer therapy; gene transfer; chemistry; dna; genetic engineering; glioblastoma; ovary tumor; tumor cell line; energy transfer; imaging; fluorescence spectroscopy; phantoms, imaging; tumor; surface plasmon resonance; synthesis; drug carrier; fluorescence imaging; drug carriers; electrostimulation; equipment; metal nanoparticle; metal nanoparticles; low level laser therapy; laser therapy; transmission electron microscopy; process optimization; limit of detection; optical imaging; devices; procedures; detection method; raman spectroscopy; raman spectrometry; spectrum analysis, raman; silver; photothermal therapy; ultraviolet visible spectroscopy; imaging phantom; cancer; humans; human; female; article; cell viability assay; gold nanorod; imaging method; chromophore assisted light inactivation; surface enhanced raman spectroscopy; low-level light therapy
Journal Title: Nature Communications
Volume: 10
ISSN: 2041-1723
Publisher: Nature Publishing Group  
Date Published: 2019-04-26
Start Page: 1926
Language: English
DOI: 10.1038/s41467-019-09173-2
PUBMED: 31028250
PROVIDER: scopus
PMCID: PMC6486596
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85064901739&doi=10.1038%2fs41467-019-09173-2&partnerID=40&md5=4c902b45747e07f8ddbb7b09f8682e44
Notes: Source: Scopus
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MSK Authors
  1. Moritz Florian Kircher
    55 Kircher
  2. Anton Oseledchyk
    16 Oseledchyk
  3. Chrysafis Andreou
    22 Andreou
  4. Suchetan Pal
    8 Pal
  5. Jiang Yang
    7 Yang
  6. Hsiao Ting Charlene Hsu
    14 Hsu
  7. Masatomo Maeda
    11 Maeda
  8. Naxhije Berisha
    11 Berisha
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