| Abstract: |
cDNA encoding the 20-kD subunit of coatomer, ζ-COP, predicts a protein of 177-amino acid residues, similar in sequence to AP17 and AP19, subunits of the clathrin adaptor complexes. Polyclonal antibody directed to ζ-COP blocks the binding of coatomer to Golgi membranes and prevents the assembly of COP- coated vesicles on Golgi cisternae. Unlike other coatomer subunits (β-, β'- , γ-, and ε-COP), ζ-COP exists in both coatomer bound and free pools. |
| Keywords: |
controlled study; nonhuman; polymerase chain reaction; protein localization; protein analysis; animal cell; animal; gtp-binding proteins; membrane proteins; intracellular transport; animalia; monoclonal antibody; liver; amino acid sequence; molecular sequence data; brain; carrier proteins; base sequence; dna sequence; cattle; dna primers; guanosine triphosphate; molecular weight; cytosol; complementary dna; chromatography, gel; membrane binding; dna, complementary; oligonucleotides, antisense; golgi complex; cho cell; cho cells; membrane vesicle; clathrin; intracellular membranes; guanosine 5'-o-(3-thiotriphosphate); restriction mapping; golgi apparatus; coat protein; bos taurus; guanosine 5' o (3 thiotriphosphate); priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; macromolecular systems; hamsters; rhob gtp-binding protein
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