En bloc incorporation of coatomer subunits during the assembly of COP-coated vesicles Journal Article
| Authors: | Hara-Kuge, S.; Kuge, O.; Orci, L.; Amherdt, M.; Ravazzola, M.; Wieland, F. T.; Rothman, J. E. |
| Article Title: | En bloc incorporation of coatomer subunits during the assembly of COP-coated vesicles |
| Abstract: | The cDNA encoding ε-COP, the 36-kD subunit of coatomer, was cloned from a bovine liver cDNA library and sequenced. Immunoblotting with an anti-ε-COP antibody showed that ε-COP exists in COP-coated vesicles as well as in the cytosolic coatomer. Using the cloned cDNA, recombinant His6-tagged ε-COP was overexpressed in cultured Chinese hamster ovary (CHO) cells, from which metabolically radiolabeled coatomer was purified by taking advantage of the His6 tag. Radiolabeled coatomer was employed to establish that all the subunits of the coatomer enter coated vesicles as an intact unit. |
| Keywords: | nonhuman; animal cell; animal; animal tissue; gene expression; membrane proteins; transfection; molecular cloning; cloning, molecular; amino acid sequence; molecular sequence data; protein purification; protein transport; immunoblotting; base sequence; dna sequence; cattle; antibody affinity; polyacrylamide gel electrophoresis; stoichiometry; organelles; cho cell; cho cells; cricetinae; cricetulus griseus; rabbits; immunoelectron microscopy; bovinae; coatomer protein; dna library; golgi apparatus; gel filtration; microscopy, immunoelectron; priority journal; article; support, non-u.s. gov't; hamsters |
| Journal Title: | Journal of Cell Biology |
| Volume: | 124 |
| Issue: | 6 |
| ISSN: | 0021-9525 |
| Publisher: | Rockefeller University Press |
| Date Published: | 1994-03-15 |
| Start Page: | 883 |
| End Page: | 892 |
| Language: | English |
| PROVIDER: | scopus |
| PMCID: | PMC2119964 |
| PUBMED: | 8132710 |
| DOI: | 10.1083/jcb.124.6.883 |
| DOI/URL: | |
| Notes: | Export Date: 14 January 2019 -- Article -- Source: Scopus |
