| Abstract: |
A genomic clone encoding prothymosin α (gene symbol: PTMA), a nuclear-targeted protein associated with cell proliferation, was isolated and the 5′-regulatory region subcloned and sequenced. Because of previously reported discrepancies between several cDNA clones and a genomic clone for prothymosin α, we determined the sequence of the first exon and of a 1.7-kb region 5′ to the first exon. The sequence of the genomic clone reported here corresponds to the published cDNA sequences, suggesting that the previously noted discrepancies may be due to genetic polymorphism in this region. In addition, our sequence data extend the known 5′-upstream sequence by an additional 1.5 kb allowing the identification of numerous, potential cis-acting regulatory sites. This 5′-flanking cloned probe permitted us to localize the protothymosin gene to chromosome 2 in humans. © 1993 Springer-Verlag. |
| Keywords: |
human cell; promoter region; exon; nonhuman; animal cell; mouse; gene; chromosomes, human, pair 2; molecular cloning; cloning, molecular; molecular sequence data; base sequence; dna flanking region; dna sequence; nucleic acid hybridization; thymosin; chromosome 2; repetitive sequences, nucleic acid; gene structure; genetic polymorphism; chromosomal localization; sequence analysis, dna; protein precursors; genetic organization; promoter regions (genetics); human; priority journal; article; polymorphism (genetics); support, non-u.s. gov't; support, u.s. gov't, p.h.s.; deoxyribonuclease bamhi; hormone precursor
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