Effects of caloric restriction and dietary fat on epithelial cell proliferation in rat colon Journal Article


Authors: Steinbach, G.; Kumar, S. P.; Reddy, B. S.; Lipkin, M.; Holt, P. R.
Article Title: Effects of caloric restriction and dietary fat on epithelial cell proliferation in rat colon
Abstract: Epidemiological studies indicate that caloric intake and dietary fat content influence colonic carcinogenesis. In rodents, caloric restriction reduces, and some fats increase, carcinogen-induced colon cancer incidence. The present study was designed to investigate the effects of caloric restriction on colonic cell proliferation (CCP) in carcinogen-treated or control rats fed low- or high-fat diets. F344 rats were treated with azoxymethane (15 mg/kg ×2) and then fed an isocaloric AIN 76A diet containing either 5 or 23% corn oil, ad libitum or calorie-restricted to 70 or 80% of the kilocalories consumed by ad libitum rats. Biopsies of the distal colon were taken at 10 and 20 weeks, and rats were sacrificed at 21 or 54 weeks on the experimental diets. Distal CCP was determined by microautoradiography after [3H]thymidine labeling in vitro or presacrifice administration in vivo. The labeling index and number of labeled cells per crypt column were significantly reduced by caloric restriction at all time points (10,20,21,34 weeks). Caloric restriction reduced CCP in high fat-and low fat-fed rats and in azoxymethane-treated and control rats. High fat resulted in decreased CCP in the distal colon compared to low fat at 34 weeks but not earlier. The findings indicate that: (a) caloric restriction is effective in favorably modulating CCP, an intermediate biomarker of colon cancer risk; (b) a high fat ad libitum diet, which increased tumor yield, does not increase distal colon proliferation; (c) dietary fat intake alters proliferation in a manner differing from that induced by changing dietary caloric intake. © 1993, American Association for Cancer Research. All rights reserved.
Keywords: controlled study; cancer localization; cancer growth; cancer risk; nonhuman; cancer incidence; energy intake; cell proliferation; animal cell; animal; animal tissue; cell division; animal experiment; colonic neoplasms; rat; epithelial cells; rats; colon; colon carcinogenesis; colon biopsy; rats, inbred f344; fat intake; crypt cell; caloric restriction; male; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; azoxymethane; corn oil
Journal Title: Cancer Research
Volume: 53
Issue: 12
ISSN: 0008-5472
Publisher: American Association for Cancer Research  
Date Published: 1993-06-15
Start Page: 2745
End Page: 2749
Language: English
PUBMED: 8504415
PROVIDER: scopus
DOI/URL:
Notes: Source: Scopus
Citation Impact
MSK Authors
  1. Martin   Lipkin
    116 Lipkin