Resolution of creatine and phosphocreatine 1H signals in isolated human skeletal muscle using HR-MAS 1H NMR Journal Article
| Authors: | Chen, J. H.; Wu, Y. V.; Decarolis, P.; O'Connor, R.; Somberg, C. J.; Singer, S. |
| Article Title: | Resolution of creatine and phosphocreatine 1H signals in isolated human skeletal muscle using HR-MAS 1H NMR |
| Abstract: | Proton NMR spectra of freshly isolated human skeletal muscle samples contain creatine and phosphocreatine resonances with distinct chemical shifts that are easily visualized with magic angle spinning (MAS, spinning the sample rapidly at 54.7° with respect to the magnetic field) methods. The identification of the phosphocreatine resonance was based on two findings: that (i) the possible small dipolar coupling does not contribute to line splitting under rapid MAS, and (ii) the 1H signal decreases concurrently with the phosphocreatine resonance observed in 31P NMR experiments. In the MAS 1H spectra, the phosphocreatine resonance remains a singlet with a linewidth of less than 3 Hz. The creatine resonances are split into two peaks with linewidths at half height of approximately 2 and 6 Hz, respectively. The resonance with the broader linewidth represents creatine that is significantly motion-restricted and suggests that a creatine pool in muscle tissue is highly compartmentalized. © 2008 Wiley-Liss, Inc. |
| Keywords: | human tissue; creatinine; magnetic resonance spectroscopy; creatine; motion; proton nuclear magnetic resonance; skeletal muscle; muscle, skeletal; creatine phosphate; hr-mas 1h nmr; resolution of creatine and phosphocreatine; magnetic field; muscle tissue; phosphocreatine |
| Journal Title: | Magnetic Resonance in Medicine |
| Volume: | 59 |
| Issue: | 6 |
| ISSN: | 0740-3194 |
| Publisher: | John Wiley & Sons |
| Date Published: | 2008-06-01 |
| Start Page: | 1221 |
| End Page: | 1224 |
| Language: | English |
| DOI: | 10.1002/mrm.21604 |
| PUBMED: | 18506783 |
| PROVIDER: | scopus |
| PMCID: | PMC2694043 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 3" - "Export Date: 17 November 2011" - "CODEN: MRMEE" - "Source: Scopus" |
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