High-resolution MAS NMR spectroscopy detection of the spin magnetization exchange by cross-relaxation and chemical exchange in intact cell lines and human tissue specimens Journal Article
| Authors: | Chen, J. H.; Sambol, E. B.; Decarolis, P.; O'Connor, R.; Geha, R. C.; Wu, Y. V.; Singer, S. |
| Article Title: | High-resolution MAS NMR spectroscopy detection of the spin magnetization exchange by cross-relaxation and chemical exchange in intact cell lines and human tissue specimens |
| Abstract: | High-resolution magic-angle-spinning (HR-MAS) NMR spectroscopy detects resolved signals from membrane phospholipids and proteins in intact cell and tissue samples. MAS has the additional advantage of quenching spin-diffusion through a mutual "flip-flop" of neighbor spins by time-independent dipolar coupling as long as the dipolar coupling is "inhomogeneous." Under MAS, significant magnetization transfer (MT) was observed between water and each proton site in membrane phospholipid and between water and the NMR-observable protein proton signals. The MT rates between water and membrane phospholipids are lower than those between water and protein proton signals. The interaction of water to other small molecules is selective with the observation of MT from water to creatine, lactate, taurine, and glycine, but not to triglyceride, phosphocholine, choline, or myo-inositol. HR-MAS NMR allows the detection of a complete MT network between water and each proton group of creatine. Two creatine pools (one motion-restricted and one motion-free) were identified in skeletal muscle. © 2006 Wiley-Liss, Inc. |
| Keywords: | human tissue; human cell; nuclear magnetic resonance imaging; sensitivity and specificity; reproducibility of results; gastrointestinal stromal tumor; cell line; membrane proteins; mathematical model; device; membrane protein; cell membrane; magnetic resonance spectroscopy; choline; creatine; nuclear magnetic resonance spectroscopy; triacylglycerol; water; spin labels; nerve sheath tumor; inositol; glycine; tissue; skeletal muscle; cell strain hct116; phospholipids; phosphorylcholine; lactic acid; cell; membrane phospholipid; nuclear overhauser effect; creatine pools; hr-mas nmr; spin magnetisation exchange; water creatine noe; water lipid noe; taurine; spin magnetization exchange |
| Journal Title: | Magnetic Resonance in Medicine |
| Volume: | 55 |
| Issue: | 6 |
| ISSN: | 0740-3194 |
| Publisher: | John Wiley & Sons |
| Date Published: | 2006-06-01 |
| Start Page: | 1246 |
| End Page: | 1256 |
| Language: | English |
| DOI: | 10.1002/mrm.20889 |
| PUBMED: | 16676334 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 15" - "Export Date: 4 June 2012" - "CODEN: MRMEE" - "Source: Scopus" |
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