The second κB element, κB(2), of the HLA-A class I regulatory complex is an essential part of the promoter Journal Article
| Authors: | Mansky, P.; Brown, W. M.; Park, J. H.; Choi, J. W.; Yang, S. Y. |
| Article Title: | The second κB element, κB(2), of the HLA-A class I regulatory complex is an essential part of the promoter |
| Abstract: | The MHC class I regulatory complex (CRC) contains the well characterized palindromic κB element, κB1, to which a number of nuclear factors are known to bind. This cis element plays an important role in controlling the transcription of MHC class I genes. In addition, the CRC contains a second κB element, κB2, which is located in tandem with the κB1 site, 5 bp upstream. In this study, from reporter gene transfection experiments, we present evidence that this κB2 site is important as κB1 in regulating HLA-A locus transcription. Mutations introduced into either κB site reduced promoter activity to approximately the level obtained when the entire CRC was deleted. Electrophoretic mobility shift and supershift assays showed that the κB2-binding complex (BIII) contained a p65-like subunit, but apparently not the p50 subunit of NF-κB. Complex BIII also bound to the κB1 site, but the κB2 sequence was poorly recognized by the κB1-binding complex, BI, probably KBF1, the p50 homodimer. These results demonstrate that both κB sites are essential components of the promoter and suggest that they may function together to control MHC class I gene transcription. |
| Keywords: | controlled study; dna binding protein; human cell; mutation; dna-binding proteins; gene; protein binding; immunoglobulin enhancer binding protein; genetic transcription; transcription, genetic; hela cells; transfection; dna; molecular sequence data; base sequence; binding site; binding sites; dna binding; hla a antigen; repetitive sequences, nucleic acid; electrophoresis; electrophoretic mobility; potassium; major histocompatibility antigen class 1; hla-a2 antigen; promoter regions (genetics); human; priority journal; article; chloramphenicol acetyltransferase; support, u.s. gov't, p.h.s. |
| Journal Title: | Journal of Immunology |
| Volume: | 153 |
| Issue: | 11 |
| ISSN: | 0022-1767 |
| Publisher: | The American Association of Immunologists, Inc |
| Date Published: | 1994-12-01 |
| Start Page: | 5082 |
| End Page: | 5090 |
| Language: | English |
| PROVIDER: | scopus |
| PUBMED: | 7963567 |
| DOI/URL: | |
| Notes: | Article -- Source: Scopus |
