DNA‐binding proteins for transcription enhancing region of HLA class I gene Journal Article

  


Authors: Park, J. H.; Lee, H. W.; Fleischhauer, K. L.; Kim, C. G.; Sheffery, M.; Yang, S. Y.
Article Title: DNA‐binding proteins for transcription enhancing region of HLA class I gene
Abstract: Abstract: Class I regulatory complex (CRC) located in the 5′‐upstream region of MHC class I gene contains transcriptional enhancing sequences, called Enh A. This Enh A region contains tandem‐arranged κB‐like sites, one of which has a well‐conserved perfect palindromic sequence. The second κB‐like site, juxtaposed to the perfect palindrome, contains an imperfect palindromic sequence. In B‐cell nuclear extracts, we have identified at least four sequence‐specific protein complexes; three shared the repeated κB enhancer as their binding motifs. The perfect palindromic sequence facilitates the binding of a complex termed BI, while the imperfect palindrome provides the binding sites for two other complexes, BII and BIII. The BII and BIII complexes exhibited binding crossreactivity with other κB‐related motifs and recognized both the perfect and imperfect palindromic sequences, whereas the BI complex was specific for the perfect palindromic sequence which is unique to the class I promoters. A DNA segment outside the repeated κB enhancers probably binds the fourth complex, BIV. These complexes, except for the perfect palindrome‐binding complex, differ from those described for the murine class I promoter. The binding characteristics of these factors suggest that the mechanism controlling the class I transcription may be quite complex. 1993 Blackwell Munksgaard
Keywords: controlled study; dna binding protein; human cell; promoter region; dna-binding proteins; animal; mice; genetic transcription; transcription, genetic; b lymphocyte; gene expression regulation; molecular sequence data; nf-kappa b; hla antigen class 1; base sequence; binding site; cell nucleus; cross reaction; dna binding; hla antigens; hla system; enhancer region; regulatory sequences, nucleic acid; enhancer elements (genetics); palindromic dna; genes, mhc class i; h-2 antigens; human; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; histocompatibility gene; hla class i regulation – nuclear complexes –κb enhancers; leukocyte extract
Journal Title: Tissue Antigens
Volume: 42
Issue: 2
ISSN: 0001-2815
Publisher: Wiley-Blackwell Publishing, Inc.  
Date Published: 1993-08-01
Start Page: 78
End Page: 86
Language: English
DOI: 10.1111/j.1399-0039.1993.tb02171.x
PUBMED: 8266321
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027377428&doi=10.1111%2fj.1399-0039.1993.tb02171.x&partnerID=40&md5=eb0a7c31707f33b4f213f844ade102d6
Notes: Article -- Export Date: 1 March 2019 -- Source: Scopus
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MSK Authors
  1. Soo Y Yang
    108 Yang
  2. Michael B. Sheffery
    23 Sheffery
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