The pentanucleotide ATTGG, the "inverted CCAAT," is an essential element for HLA class I gene transcription Journal Article
| Authors: | Schoneich, J.; Lee, J. L.; Mansky, P.; Sheffery, M.; Yang, S. Y. |
| Article Title: | The pentanucleotide ATTGG, the "inverted CCAAT," is an essential element for HLA class I gene transcription |
| Abstract: | The HLA class I genes, HLA-A, -B, and -C, contain an inverted CCAAT sequence (ATTGG) located 20 bp upstream of the canonical CCAAT and approximately 70 bp upstream of the transcription initiation site. We have investigated the transcriptional function of the class I inverted CCAAT sequence using the HLA-normal cell line, HeLa. Deletion, mutation, or inversion of the inverted CCAAT sequence abrogated or reduced the activity of the class I promoter, as assessed by luciferase reporter gene assays in transient gene expression experiments. This activity coincided with occupancy of the inverted CCAAT motif, as tested by electrophoretic mobility shift assays using the wild-type sequence and mutated variants of the sequence. The ATTGG-binding protein was not CP2, NF-1, or other known CCAAT-binding proteins, but the complex may contain a CP1/NF-Y-like protein. Our results indicate that this inverted CCAAT sequence is an essential element for the expression of HLA class I genes and that its transcriptional activity depends upon the sequence, position, and orientation of the pentanucleotide. |
| Keywords: | dna binding protein; promoter region; genetics; dna-binding proteins; molecular genetics; gene; nuclear protein; genetic transcription; transcription, genetic; physiology; nuclear proteins; chemistry; molecular sequence data; sequence alignment; nucleotide sequence; hla antigen class 1; histocompatibility antigens class i; base sequence; binding site; binding sites; sequence homology; sequence homology, nucleic acid; ccaat-enhancer-binding proteins; promoter regions (genetics); genes, mhc class i; ccaat enhancer binding protein; humans; human; article |
| Journal Title: | Journal of Immunology |
| Volume: | 158 |
| Issue: | 10 |
| ISSN: | 0022-1767 |
| Publisher: | The American Association of Immunologists, Inc |
| Date Published: | 1997-05-15 |
| Start Page: | 4788 |
| End Page: | 4796 |
| Language: | English |
| PUBMED: | 9144493 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |
Citation Impact
Related MSK Work