| Abstract: |
A B cell lymphoma-associated chromosomal translocation, t(10;14)(q24;q32), juxtaposes the immunoglobulin Cα1 locus to a novel gene, lyt-10. The normal lyt-10 cDNA codes for a 98 kd protein which displays amino-terminal homology with the rel (DNA-binding) domain of the NF-κB-rel family of transcription factors and carboxy-terminal homology with the NF-κB p50 precursor protein, including the putative proteolytic cleavage domain (poly-G) and the ankyrin-like repeat domains. The lyt-10 protein can bind to κB sequences in vitro, although with different specificity from NF-κB p50, and in vitro DNA-binding is activated by removal of the ankyrin domain. Chromosomal translocation generates an lyt-10-Cα1 fusion gene coding for a protein that retains the rel effector domain, lacks the ankyrin regulatory domain, and binds κB sequences in vitro, suggesting its constitutive activation in vivo. Analogous rearrangements of the lyt-10 gene have been found in an additional three cases of lymphoid neoplasia. The lyt-10 gene defines a new subfamily (rel/poly-G/ankyrin) of NF-κB-rel transcription factors with potential for oncogenic activation in human cancer. © 1991. |
| Keywords: |
human cell; dna-binding proteins; review; polymerase chain reaction; gene expression; gene locus; transcription factor; immunoglobulin; transcription factors; oncogenes; cloning, molecular; oncogene; b cell lymphoma; lymphoma, b-cell; dna; immunoglobulin gene; amino acid sequence; molecular sequence data; amino terminal sequence; rna, messenger; nucleotide sequence; nf-kappa b; chromosome translocation; base sequence; sequence homology; dna binding; multigene family; oligodeoxyribonucleotides; regulatory sequences, nucleic acid; restriction mapping; chromosomes, human, pair 14; genes, immunoglobulin; ankyrin; chromosomes, human, pair 10; gene rearrangement, b-lymphocyte, heavy chain; human; priority journal; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; translocation (genetics); immunoglobulins, alpha-chain
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