The transcriptional activity of NF-κB is regulated by the IκB- associated PKAc subunit through a cyclic AMP-independent mechanism Journal Article
| Authors: | Zhong, H.; Su Yang, H.; Erdjument-Bromage, H.; Tempst, P.; Ghosh, S. |
| Article Title: | The transcriptional activity of NF-κB is regulated by the IκB- associated PKAc subunit through a cyclic AMP-independent mechanism |
| Abstract: | Stimulation of cells with inducers of NF-κB such as LPS and IL-1 leads to the degradation of IκB-α and IκB-β proteins and translocation of NF- κB to the nucleus. We now demonstrate that, besides the physical partitioning of inactive NF-κB to the cytosol, the transcriptional activity of NF-κB is regulated through phosphorylation of NF-κB p65 by protein kinase A (PKA). The catalytic subunit of PKA (PKAc) is maintained in an inactive state through association with IκB-α or IκB-̄ in an NF-κB- IκB-PKAc complex. Signals that cause the degradation of IκB result in activation of PKAc in a cAMP-independent manner and the subsequent phosphorylation of p65. Therefore, this pathway represents a novel mechanism for the cAMP-independent activation of PKA and the regulation of NF-≃B activity. |
| Keywords: | controlled study; protein phosphorylation; dna-binding proteins; nonhuman; animal cell; animals; protein binding; transcription factor; immunoglobulin enhancer binding protein; enzyme activation; enzyme activity; phosphorylation; animalia; enzyme regulation; transcription regulation; amino acid sequence; molecular sequence data; enzyme inhibitors; nf-kappa b; lung; cyclic amp; lipopolysaccharide; protein structure, tertiary; cell nucleus; adenosine triphosphate; cytosol; synaptotagmin; regulator protein; enzyme active site; interleukin 1; gene expression regulation, enzymologic; cyclic amp dependent protein kinase; cyclic amp-dependent protein kinases; rabbits; lipopolysaccharides; rabbit; trans-activation (genetics); ankyrin; oryctolagus cuniculus; calpain; priority journal; article |
| Journal Title: | Cell |
| Volume: | 89 |
| Issue: | 3 |
| ISSN: | 0092-8674 |
| Publisher: | Cell Press |
| Date Published: | 1997-05-02 |
| Start Page: | 413 |
| End Page: | 424 |
| Language: | English |
| DOI: | 10.1016/s0092-8674(00)80222-6 |
| PUBMED: | 9150141 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |
