A positively selecting thymic epithelial cell line lacks costimulatory activity Journal Article
| Authors: | Vukmanović, S.; Stella, G.; King, P. D.; Dyall, R.; Hogquist, K. A.; Harty, J. T.; Nikolić-Žugić, J.; Bevan, M. J. |
| Article Title: | A positively selecting thymic epithelial cell line lacks costimulatory activity |
| Abstract: | The participation of costimulatory molecule interactions in positive selection of T lymphocytes was addressed by assessing the ability of a positively selecting thymic epithelial cell (TEC) line, 427.1, to stimulate allospecific CTL responses. Stimulation of H-2(s) spleen cells with the H- 2b expressing 427.1 line does not result in the generation of cells capable of lysing H-2b target cell lines. The level of expression of MHC class I molecules by 427.1 is lower than that found in other stimulatory TEC lines. However, this finding does not account for the nonstimulatory phenotype. Up- regulation of MHC class I did not result in stimulation, and fusion of 427.1 cells with stimulatory TEC resulted in a line with low MHC class I molecule expression and stimulatory phenotype. The TEC line 427.1 does not express the costimulatory molecule B7/BB1, and transfection of the B7/BB1-encoding DNA results in expression of the molecule and conversion into a stimulatory phenotype, demonstrating directly that the non-stimulatory phenotype is a result of lack of costimulation. However, B7/BB1 expression does not improve the ability of 427.1 TECs to induce positive selection. Intrathymic injection of the B7/BB1 transfected, compared with mock transfected 427.1 cells, rescued fewer CD8+ mature thymocytes in β2-microglobulin negative mice. Therefore, unlike the peripheral T cell responses to Ag, positive selection of T cells in the thymus may not depend on the costimulation provided by the presenting cell. |
| Keywords: | nonhuman; cd8 antigen; antigen expression; t-lymphocytes; animal cell; mouse; animals; mice; cell line; mice, inbred strains; molecular cloning; t lymphocyte receptor; antigen presentation; dna; cellular immunity; molecular sequence data; thymus gland; cytotoxic t lymphocyte; epithelial cells; base sequence; effector cell; target cell; cytotoxicity, immunologic; cd4 antigen; dna primers; thymocyte; antigen-presenting cells; cell selection; complementary dna; major histocompatibility antigen class 1; alloimmunity; antigens, cd80; h-2 antigens; priority journal; article |
| Journal Title: | Journal of Immunology |
| Volume: | 152 |
| Issue: | 8 |
| ISSN: | 0022-1767 |
| Publisher: | The American Association of Immunologists, Inc |
| Date Published: | 1994-04-15 |
| Start Page: | 3814 |
| End Page: | 3823 |
| Language: | English |
| PROVIDER: | scopus |
| PUBMED: | 7511660 |
| DOI/URL: | |
| Notes: | Article -- Source: Scopus |
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