The Escherichia coli replisome is inherently DNA damage tolerant Journal Article
| Authors: | Yeeles, J. T. P.; Marians, K. J. |
| Article Title: | The Escherichia coli replisome is inherently DNA damage tolerant |
| Abstract: | The Escherichia coli DNA replication machinery must frequently overcome template lesions under normal growth conditions. Yet, the outcome of a collision between the replisome and a leading-strand template lesion remains poorly understood. Here, we demonstrate that a single, site-specific, cyclobutane pyrimidine dimer leading-strand template lesion provides only a transient block to fork progression in vitro. The replisome remains stably associated with the fork after collision with the lesion. Leading-strand synthesis is then reinitiated downstream of the damage in a reaction that is dependent on the primase, DnaG, but independent of any of the known replication-restart proteins. These observations reveal that the replisome can tolerate leading-strand template lesions without dissociating by synthesizing the leading strand discontinuously. |
| Keywords: | dna binding protein; nonhuman; dna replication; dna damage; cell survival; protein; enzyme activity; dna; double stranded dna; escherichia coli; plasmid; plasmids; coliform bacterium; single stranded dna; dna topoisomerase (atp hydrolysing); multienzyme complexes; dna, bacterial; pyrimidine; escherichia coli proteins; endodeoxyribonucleases; exodeoxyribonucleases; pyrimidine dimers; lesion; dna-directed dna polymerase; dimer; replisome; bacterial cell; dna directed dna polymerase gamma; templates, genetic; cyclobutane; replication origin; dna polymerase iii |
| Journal Title: | Science |
| Volume: | 334 |
| Issue: | 6053 |
| ISSN: | 0036-8075 |
| Publisher: | American Association for the Advancement of Science |
| Date Published: | 2011-10-14 |
| Start Page: | 235 |
| End Page: | 238 |
| Language: | English |
| DOI: | 10.1126/science.1209111 |
| PROVIDER: | scopus |
| PUBMED: | 21998391 |
| PMCID: | PMC3593629 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 2 November 2011" - "CODEN: SCIEA" - "Source: Scopus" |
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