Replisome bypass of transcription complexes and R-loops Journal Article
| Authors: | Brüning, J. G.; Marians, K. J. |
| Article Title: | Replisome bypass of transcription complexes and R-loops |
| Abstract: | The vast majority of the genome is transcribed by RNA polymerases. G+C-rich regions of the chromosomes and negative superhelicity can promote the invasion of the DNA by RNA to form R-loops, which have been shown to block DNA replication and promote genome instability. However, it is unclear whether the R-loops themselves are sufficient to cause this instability or if additional factors are required. We have investigated replisome collisions with transcription complexes and R-loops using a reconstituted bacterial DNA replication system. RNA polymerase transcription complexes co-directionally oriented with the replication fork were transient blockages, whereas those oriented head-on were severe, stable blockages. On the other hand, replisomes easily bypassed R-loops on either template strand. Replication encounters with R-loops on the leading-strand template (co-directional) resulted in gaps in the nascent leading strand, whereas lagging-strand template R-loops (head-on) had little impact on replication fork progression. We conclude that whereas R-loops alone can act as transient replication blocks, most genome-destabilizing replication fork stalling likely occurs because of proteins bound to the R-loops. © The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research. |
| Journal Title: | Nucleic Acids Research |
| Volume: | 48 |
| Issue: | 18 |
| ISSN: | 0305-1048 |
| Publisher: | Oxford University Press |
| Date Published: | 2020-10-09 |
| Start Page: | 10353 |
| End Page: | 10367 |
| Language: | English |
| DOI: | 10.1093/nar/gkaa741 |
| PUBMED: | 32926139 |
| PROVIDER: | scopus |
| PMCID: | PMC7544221 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 2 November 2020 -- Source: Scopus |
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