Regression of replication forks stalled by leading-strand template damage: I. Both RecG and RuvAB catalyze regression, but RuvC cleaves the Holliday junctions formed by RecG preferentially Journal Article


Authors: Gupta, S.; Yeeles, J. T. P.; Marians, K. J.
Article Title: Regression of replication forks stalled by leading-strand template damage: I. Both RecG and RuvAB catalyze regression, but RuvC cleaves the Holliday junctions formed by RecG preferentially
Abstract: The orderly progression of replication forks formed at the origin of replication in Escherichia coli is challenged by encounters with template damage, slow moving RNA polymerases, and frozen DNA-protein complexes that stall the fork. These stalled forks are foci for genomic instability and must be reactivated. Many models of replication fork reactivation invoke nascent strand regression as an intermediate in the processing of the stalled fork. We have investigated the replication fork regression activity of RecG and RuvAB, two proteins commonly thought to be involved in the process, using a reconstituted DNA replication system where the replisome is stalled by collision with leading-strand template damage. We find that both RecG and RuvAB can regress the stalled fork in the presence of the replisome and SSB; however, RuvAB generates a completely unwound product consisting of the paired nascent leading and lagging strands, whereas RuvC cleaves the Holliday junction generated by RecG-catalyzed fork regression. We also find that RecG stimulates RuvAB-catalyzed regression, presumably because it is more efficient at generating the initial Holliday junction from the stalled fork.
Keywords: unclassified drug; nonhuman; dna replication; bacterial protein; dna; genomic instability; escherichia coli; catalysis; replication fork; recg helicase; replisome; nucleic acid structure, metabolism and function; holliday junction; article; holliday junctions; protein ruvab; replication fork regression
Journal Title: Journal of Biological Chemistry
Volume: 289
Issue: 41
ISSN: 0021-9258
Publisher: American Society for Biochemistry and Molecular Biology  
Date Published: 2014-10-10
Start Page: 28376
End Page: 28387
Language: English
DOI: 10.1074/jbc.M114.587881
PROVIDER: scopus
PMCID: PMC4192490
PUBMED: 25138216
DOI/URL:
Notes: Export Date: 3 November 2014 -- Source: Scopus
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  1. Joseph Thomas Pinkerton Yeeles
    7 Yeeles
  2. Sankalp Gupta
    3 Gupta
  3. Kenneth Marians
    138 Marians