Dynamics of leading-strand lesion skipping by the replisome Journal Article
| Authors: | Yeeles, J. T. P.; Marians, K. J. |
| Article Title: | Dynamics of leading-strand lesion skipping by the replisome |
| Abstract: | The E.coli replisome stalls transiently when it encounters a lesion in the leading-strand template, skipping over the damage by reinitiating replication at a new primer synthesized downstream by the primase. We report here that template unwinding and lagging-strand synthesis continue downstream of the lesion at a reduced rate after replisome stalling, that one replisome is capable of skipping multiple lesions, and that the rate-limiting steps of replication restart involve the synthesis and activation of the new primer downstream. We also find little support for the concept that polymerase uncoupling, where extensive lagging-strand synthesis proceeds downstream in the absence of leading-strand synthesis, involves physical separation of the leading-strand polymerase from the replisome. Instead, our data indicate that extensive uncoupled replication likely results from a failure of the leading-strand polymerase still associated with the DNA helicase and thelagging-strand polymerase that are proceeding downstream to reinitiate synthesis. © 2013 Elsevier Inc. |
| Journal Title: | Molecular Cell |
| Volume: | 52 |
| Issue: | 6 |
| ISSN: | 1097-2765 |
| Publisher: | Cell Press |
| Date Published: | 2013-12-26 |
| Start Page: | 855 |
| End Page: | 865 |
| Language: | English |
| DOI: | 10.1016/j.molcel.2013.10.020 |
| PROVIDER: | scopus |
| PMCID: | PMC3877186 |
| PUBMED: | 24268579 |
| DOI/URL: | |
| Notes: | Export Date: 3 February 2014 -- CODEN: MOCEF -- Source: Scopus |
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