PPP1R35 ensures centriole homeostasis by promoting centriole-to-centrosome conversion Journal Article

Authors: Fong, C. S.; Ozaki, K.; Tsou, M. F. B.
Article Title: PPP1R35 ensures centriole homeostasis by promoting centriole-to-centrosome conversion
Abstract: Centriole-to-centrosome conversion (CCC) safeguards centriole homeostasis by coupling centriole duplication with segregation, and is essential for stabilization of mature vertebrate centrioles naturally devoid of the geometric scaffold or the cartwheel. Here we identified PPP1R35, a putative regulator of the protein phosphatase PP1, as a novel centriolar protein required for CCC. We found that PPP1R35 is enriched at newborn daughter centrioles in S or G2 phase. In the absence of PPP1R35, centriole assembly initiates normally in S phase, but none of the nascent centrioles can form active centrosomes or recruit CEP295, an essential factor for CCC. Instead, all PPP1R35-null centrioles, although stable during their birth in interphase, become disintegrated after mitosis upon cartwheel removal. Surprisingly, we found that neither the centriolar localization nor the function of PPP1R35 in CCC requires the putative PP1-interacting motif. PPP1R35 is thus acting upstream of CEP295 to induce CCC for proper centriole maintenance. © 2018 Fong et al.
Journal Title: Molecular Biology of the Cell
Volume: 29
Issue: 23
ISSN: 1059-1524
Publisher: The American Society for Cell Biology  
Date Published: 2018-11-15
Start Page: 2801
End Page: 2808
Language: English
DOI: 10.1091/mbc.E18-08-0525
PUBMED: 30230954
PROVIDER: scopus
PMCID: PMC6249868
Notes: Article -- Export Date: 3 December 2018 -- Source: Scopus
Citation Impact
MSK Authors
  1. Meng-Fu Bryan Tsou
    30 Tsou
  2. Chii Shyang Fong
    10 Fong
  3. Kanako Ozaki
    1 Ozaki