Stabilization of cartwheel-less centrioles for duplication requires CEP295-mediated centriole-to-centrosome conversion Journal Article


Authors: Izquierdo, D.; Wang, W. J.; Uryu, K.; Tsou, M. F. B.
Article Title: Stabilization of cartwheel-less centrioles for duplication requires CEP295-mediated centriole-to-centrosome conversion
Abstract: Vertebrate centrioles lose their geometric scaffold, the cartwheel, during mitosis, concurrently with gaining the ability to recruit the pericentriolar material (PCM) and thereby function as the centrosome. Cartwheel removal has recently been implicated in centriole duplication, but whether "cartwheel-less" centrioles are intrinsically stable or must be maintained through other modifications remains unclear. Here, we identify a newborn centriole-enriched protein, KIAA1731/CEP295, specifically mediating centriole-to-centrosome conversion but dispensable for cartwheel removal. In the absence of CEP295, centrioles form in the S/G2 phase and lose their associated cartwheel in mitosis but cannot be converted to centrosomes, uncoupling the two events. Strikingly, centrioles devoid of both the PCM and the cartwheel progressively lose centriolar components, whereas centrioles associating with either the cartwheel or PCM alone can exist stably. Thus, cartwheel removal can have grave repercussions to centriole stability, and centriole-to-centrosome conversion mediated by CEP295 must occur in parallel to maintain cartwheel-less centrioles for duplication. © 2014 The Authors.
Keywords: controlled study; unclassified drug; human cell; mitosis; protein analysis; cell cycle s phase; cell function; cell structure; protein assembly; protein depletion; cell protein; vertebrata; cell cycle g2 phase; vertebrate; protein synthesis inhibition; centriole; centrosome; procedures concerning cells; cellular parameters; human; priority journal; article; cep295 protein; cartwheel less centriole; cartwheel removal; centriole duplication; centriole to centrosome conversion; pericentriolar region
Journal Title: Cell Reports
Volume: 8
Issue: 4
ISSN: 2211-1247
Publisher: Cell Press  
Date Published: 2014-08-21
Start Page: 957
End Page: 965
Language: English
DOI: 10.1016/j.celrep.2014.07.022
PROVIDER: scopus
PUBMED: 25131205
PMCID: PMC4152953
DOI/URL:
Notes: Export Date: 1 December 2014 -- Source: Scopus
Altmetric
Citation Impact
MSK Authors
  1. Won-Jing Wang
    12 Wang
  2. Meng-Fu Bryan Tsou
    30 Tsou