Centriole distal appendages promote membrane docking, leading to cilia initiation Journal Article

Authors: Tanos, B. E.; Yang, H. J.; Soni, R.; Wang, W. J.; Macaluso, F. P.; Asara, J. M.; Tsou, M. F. B.
Article Title: Centriole distal appendages promote membrane docking, leading to cilia initiation
Abstract: The distal appendages (DAPs) of centrioles have been proposed to anchor cilia to the plasma membrane, but their molecular composition, assembly, and exact function in ciliogenesis remain poorly understood. Using quantitative centrosome proteomics and superresolution microscopy, we identified five DAP components, including one previously described (CEP164), one partially characterized (CEP89 [ccdc123]), and three novel (CEP83 [ccdc41], SCLT1, and FBF1) DAP proteins. Analyses of DAP assembly revealed a hierarchy. CEP83 recruits both SCLT1 and CEP89 to centrioles. Subsequent recruitment of FBF1 and CEP164 is independent of CEP89 but mediated by SCLT1. All five DAP components are essential for ciliogenesis; loss of CEP83 specifically blocks centriole- to-membrane docking. Undocked centrioles fail to recruit TTBK2 or release CP110, the two earliest modifications found on centrioles prior to cilia assembly, revealing centriole-to-membrane docking as a temporal and spatial cue promoting cilia initiation. © 2013 by Cold Spring Harbor Laboratory Press.
Keywords: cilia; centriole; distal appendages; transition fibers; membrane docking
Journal Title: Genes and Development
Volume: 27
Issue: 2
ISSN: 0890-9369
Publisher: Cold Spring Harbor Laboratory Press  
Date Published: 2013-01-15
Start Page: 163
End Page: 168
Language: English
DOI: 10.1101/gad.207043.112
PROVIDER: scopus
PMCID: PMC3566309
PUBMED: 23348840
Notes: --- - "Export Date: 1 April 2013" - "CODEN: GEDEE" - "Source: Scopus"
Citation Impact
MSK Authors
  1. Won-Jing Wang
    12 Wang
  2. Rajesh Kumar Soni
    16 Soni
  3. Meng-Fu Bryan Tsou
    30 Tsou
  4. Hyo Jung Yang
    3 Yang