The small GTPase RSG1 controls a final step in primary cilia initiation Journal Article
| Authors: | Agbu, S. O.; Liang, Y.; Liu, A.; Anderson, K. V. |
| Article Title: | The small GTPase RSG1 controls a final step in primary cilia initiation |
| Abstract: | Primary cilia, which are essential for normal development and tissue homeostasis, are extensions of the mother centriole, but the mechanisms that remodel the centriole to promote cilia initiation are poorly understood. Here we show that mouse embryos that lack the small guanosine triphosphatase RSG1 die at embryonic day 12.5, with developmental abnormalities characteristic of decreased cilia-dependent Hedgehog signaling. Rsg1 mutant embryos have fewer primary cilia than wild-type embryos, but the cilia that form are of normal length and traffic Hedgehog pathway proteins within the cilium correctly. Rsg1 mother centrioles recruit proteins required for cilia initiation and dock onto ciliary vesicles, but axonemal microtubules fail to elongate normally. RSG1 localizes to the mother centriole in a process that depends on tau tubulin kinase 2 (TTBK2), the CPL ANE complex protein Inturned (INTU), and its own GTPase activity. The data suggest a specific role for RSG1 in the final maturation of the mother centriole and ciliary vesicle that allows extension of the ciliary axoneme. © 2018 Agbu et al. |
| Keywords: | immunohistochemistry; controlled study; unclassified drug; microscopy; nonhuman; protein localization; animal cell; mouse; phenotype; animal tissue; reverse transcription polymerase chain reaction; embryo; rna interference; enzyme activity; wild type; genetic transfection; western blotting; developmental disorder; microtubule; protein kinase; fibroblast culture; mutant; centriole; guanosine triphosphatase; scanning electron microscopy; primary cilium; priority journal; article; hedgehog signaling; inturned protein; protein rsg1; tau tubulin kinase 2 |
| Journal Title: | Journal of Cell Biology |
| Volume: | 217 |
| Issue: | 1 |
| ISSN: | 0021-9525 |
| Publisher: | Rockefeller University Press |
| Date Published: | 2018-01-01 |
| Start Page: | 413 |
| End Page: | 427 |
| Language: | English |
| DOI: | 10.1083/jcb.201604048 |
| PROVIDER: | scopus |
| PMCID: | PMC5748968 |
| PUBMED: | 29038301 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 February 2018 -- Source: Scopus |
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