SAS-6 assembly templated by the lumen of cartwheel-less centrioles precedes centriole duplication Journal Article


Authors: Fong, C. S.; Kim, M.; Yang, T. T.; Liao, J. C.; Tsou, M. F. B.
Article Title: SAS-6 assembly templated by the lumen of cartwheel-less centrioles precedes centriole duplication
Abstract: Centrioles are 9-fold symmetric structures duplicating once per cell cycle. Duplication involves self-oligomerization of the centriolar protein SAS-6, but how the 9-fold symmetry is invariantly established remains unclear. Here, we found that SAS-6 assembly can be shaped by preexisting (or mother) centrioles. During S phase, SAS-6 molecules are first recruited to the proximal lumen of the mother centriole, adopting a cartwheel-like organization through interactions with the luminal wall, rather than via their self-oligomerization activity. The removal or release of luminal SAS-6 requires Plk4 and the cartwheel protein STIL. Abolishing either the recruitment or the removal of luminal SAS-6 hinders SAS-6 (or centriole) assembly at the outside wall of mother centrioles. After duplication, the lumen of engaged mother centrioles becomes inaccessible to SAS-6, correlating with a block for reduplication. These results lead to a proposed model that centrioles may duplicate via a template-based process to preserve their geometry and copy number. © 2014 Elsevier Inc.
Keywords: cell cycle s phase; protein assembly; protein interaction; molecular recognition; protein secretion; oligomerization; centriole; priority journal; article
Journal Title: Developmental Cell
Volume: 30
Issue: 2
ISSN: 1534-5807
Publisher: Cell Press  
Date Published: 2014-07-28
Start Page: 238
End Page: 245
Language: English
DOI: 10.1016/j.devcel.2014.05.008
PROVIDER: scopus
PMCID: PMC4116473
PUBMED: 25017693
DOI/URL:
Notes: Cited By (since 1996):1 -- Export Date: 2 September 2014 -- CODEN: DCEEB -- Source: Scopus
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MSK Authors
  1. Meng-Fu Bryan Tsou
    30 Tsou
  2. Chii Shyang Fong
    10 Fong
  3. Minhee   Kim
    6 Kim