SAS-6 assembly templated by the lumen of cartwheel-less centrioles precedes centriole duplication Journal Article

   

Authors:	Fong, C. S.; Kim, M.; Yang, T. T.; Liao, J. C.; Tsou, M. F. B.
Article Title:	SAS-6 assembly templated by the lumen of cartwheel-less centrioles precedes centriole duplication
Abstract:	Centrioles are 9-fold symmetric structures duplicating once per cell cycle. Duplication involves self-oligomerization of the centriolar protein SAS-6, but how the 9-fold symmetry is invariantly established remains unclear. Here, we found that SAS-6 assembly can be shaped by preexisting (or mother) centrioles. During S phase, SAS-6 molecules are first recruited to the proximal lumen of the mother centriole, adopting a cartwheel-like organization through interactions with the luminal wall, rather than via their self-oligomerization activity. The removal or release of luminal SAS-6 requires Plk4 and the cartwheel protein STIL. Abolishing either the recruitment or the removal of luminal SAS-6 hinders SAS-6 (or centriole) assembly at the outside wall of mother centrioles. After duplication, the lumen of engaged mother centrioles becomes inaccessible to SAS-6, correlating with a block for reduplication. These results lead to a proposed model that centrioles may duplicate via a template-based process to preserve their geometry and copy number. © 2014 Elsevier Inc.
Keywords:	cell cycle s phase; protein assembly; protein interaction; molecular recognition; protein secretion; oligomerization; centriole; priority journal; article
Journal Title:	Developmental Cell
Volume:	30
Issue:	2
ISSN:	1534-5807
Publisher:	Cell Press
Date Published:	2014-07-28
Start Page:	238
End Page:	245
Language:	English
DOI:	10.1016/j.devcel.2014.05.008
PROVIDER:	scopus
PMCID:	PMC4116473
PUBMED:	25017693
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-84904873297&partnerID=40&md5=23d64c4b43adb34f147615aec04f3281
Notes:	Cited By (since 1996):1 -- Export Date: 2 September 2014 -- CODEN: DCEEB -- Source: Scopus

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