Establishing and maintaining an extensive library of patient-derived xenograft models Journal Article

Authors: Mattar, M.; McCarthy, C. R.; Kulick, A. R.; Qeriqi, B.; Guzman, S.; de Stanchina, E.
Article Title: Establishing and maintaining an extensive library of patient-derived xenograft models
Abstract: Patient-derived xenograft (PDX) models have recently emerged as a highly desirable platform in oncology and are expected to substantially broaden the way in vivo studies are designed and executed and to reshape drug discovery programs. However, acquisition of patient-derived samples, and propagation, annotation and distribution of PDXs are complex processes that require a high degree of coordination among clinic, surgery and laboratory personnel, and are fraught with challenges that are administrative, procedural and technical. Here, we examine in detail the major aspects of this complex process and relate our experience in establishing a PDX Core Laboratory within a large academic institution. © 2018 Mattar, McCarthy, Kulick, Qeriqi, Guzman and de Stanchina.
Keywords: human tissue; leukemia; ascites; molecular genetics; genetic analysis; cell viability; tumor volume; drug development; gene library; necrosis; biopsy; histology; immune response; mononuclear cell; xenograft; blood sampling; screening; cell aggregation; pleura fluid; tumor growth; bioinformatics; pericardial effusion; cost benefit analysis; occupational health; hospital management; skin ulcer; tumor engraftment; implantation; antibody labeling; freezing; environmental health; human; article; whole genome sequencing; patient derived xenograft; patient-derived xenograft; patient sample acquisition; biobank; solid malignant neoplasm; patient-derived xenograft database; patient-derived xenograft implantation techniques; patient-derived xenograft propagation techniques
Journal Title: Frontiers in Oncology
Volume: 8
ISSN: 2234-943X
Publisher: Frontiers Media S.A.  
Date Published: 2018-02-19
Start Page: 19
Language: English
DOI: 10.3389/fonc.2018.00019
PROVIDER: scopus
PMCID: PMC5825907
PUBMED: 29515970
Notes: Article -- Export Date: 2 April 2018 -- Source: Scopus
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MSK Authors
  1. Marissa   Mattar
    8 Mattar
  2. Amanda Kulick
    4 Kulick
  3. Sean Guzman
    1 Guzman
  4. Besnik Qeriqi
    4 Qeriqi
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